Generation of a selective 5-HT3B subunit-recognising polyclonal antibody ; identification of immunoreactive cells in rat hippocampus.

Tools

MONK, S A, DESAI, K, BRADY, C A, WILLIAMS, J M, LIN, L, PRINCIVALLE, A, HOPE, A G and BARNES, N M (2001). Generation of a selective 5-HT3B subunit-recognising polyclonal antibody ; identification of immunoreactive cells in rat hippocampus. Neuropharmacology, 41 (8), 1013-1016. [Article]

Abstract
More Information
Metrics
Share

[+][-]

Abstract

The present study generated a polyclonal antibody (AP86/3) that recognises a peptide sequence of the h5-HT(3B) receptor subunit. Western blot analysis of homogenates prepared from cell lines expressing either homomeric (h5-HT(3A)) or heteromeric (h5-HT(3A/3B)) receptors, as well as immunocytochemical studies with the same cell lines, indicated that AP86/3 recognised, selectively, the 5-HT(3B) subunit. Immunohistochemical labelling was also apparent in cells in the rat hippocampus that displayed the distribution and morphology of interneurones.

More Information

Research Institute, Centre or Group - Does NOT include content added after October 2018:

Biomedical Research Centre

Page Range:

1013-1016

Identifiers

Identification Number:

10.1016/S0028-3908(01)00153-8

ORCID for A Princivalle: