Bacterial quorum sensing and cell surface electrokinetic properties

EBOIGBODIN, K. E., NEWTON, J. R. A., ROUTH, A. F. and BIGGS, C. A. (2006). Bacterial quorum sensing and cell surface electrokinetic properties. Applied Microbiology and Biotechnology, 73 (3), 669-675.

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Abstract

The hypothesis tested in this paper is that quorum sensing influences the microbial surface electrokinetic properties. Escherichia coli MG1655 and MG1655 LuxS- mutant (lacking quorum-sensing gene for Autoinducer synthase AI-2) were used for this study. AI-2 production (or lack of) in both strains was analyzed using the Vibrio harveyi bioassay. The levels of extracellular AI-2 with and without glucose in the growth medium were consistent with previously published work. The surface electrokinetic properties were determined for each strain of E. coli MG1655 by measuring the electrophoretic mobility using a phase amplitude light-scattering (PALS) Zeta potential analyser. The findings show that the surface charge of the cells is dependent upon the stage in the growth phase as well as the ability to participate in quorum sensing. In addition, significant differences in the electrophoretic mobility were observed between both strains of E. coli. These findings suggest that quorum sensing plays a significant role in the surface chemistry of bacteria during their growth.

Item Type:	Article
Research Institute, Centre or Group - Does NOT include content added after October 2018:	Biomedical Research Centre
Identification Number:	https://doi.org/10.1007/s00253-006-0505-4
Page Range:	669-675
Depositing User:	Jamie Young
Date Deposited:	28 May 2015 10:21
Last Modified:	18 Mar 2021 23:15
URI:	https://shura.shu.ac.uk/id/eprint/9896

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