Neutrophil microvesicles drive atherosclerosis by delivering miR-155 to atheroprone endothelium

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GOMEZ, Ingrid, WARD, Ben, SOUILHOL, Celine, RECARTI, Chiara, ARIAANS, Mark, JOHNSTON, Jessica, BURNETT, Amanda, MAHMOUD, Marwa, LUONG, Le Anh, WEST, Laura, LONG, Merete, PARRY, Sion, WOODS, Rachel, HULSTON, Carl, BENEDIKTER, Birke, NIESPOLO, Chiara, BAZAZ, Rohit, FRANCIS, Sheila, KISS-TOTH, Endre, VAN ZANDVOORT, Marc, SCHOBER, Andreas, HELLEWELL, Paul, EVANS, Paul C and RIDGER, Victoria (2020). Neutrophil microvesicles drive atherosclerosis by delivering miR-155 to atheroprone endothelium. Nature Communications, 11 (1): 214. [Article]

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Abstract
Neutrophils are implicated in the pathogenesis of atherosclerosis but are seldom detected in atherosclerotic plaques. We investigated whether neutrophil-derived microvesicles may influence arterial pathophysiology. Here we report that levels of circulating neutrophil microvesicles are enhanced by exposure to a high fat diet, a known risk factor for atherosclerosis. Neutrophil microvesicles accumulate at disease-prone regions of arteries exposed to disturbed flow patterns, and promote vascular inflammation and atherosclerosis in a murine model. Using cultured endothelial cells exposed to disturbed flow, we demonstrate that neutrophil microvesicles promote inflammatory gene expression by delivering miR-155, enhancing NF-κB activation. Similarly, neutrophil microvesicles increase miR-155 and enhance NF-κB at disease-prone sites of disturbed flow in vivo. Enhancement of atherosclerotic plaque formation and increase in macrophage content by neutrophil microvesicles is dependent on miR-155. We conclude that neutrophils contribute to vascular inflammation and atherogenesis through delivery of microvesicles carrying miR-155 to disease-prone regions.
More Information
Official URL:
https://doi.org/10.1038/s41467-019-14043-y

Identifiers

Identification Number:
10.1038/s41467-019-14043-y
ORCID for Mark Ariaans: ORCID iD orcid.org/0000-0002-7392-8278
ORCID for Jessica Johnston: ORCID iD orcid.org/0000-0003-4877-0680
ORCID for Laura West: ORCID iD orcid.org/0000-0003-2953-2521
ORCID for Rachel Woods: ORCID iD orcid.org/0000-0003-4524-5346
ORCID for Carl Hulston: ORCID iD orcid.org/0000-0002-5375-1161
ORCID for Birke Benedikter: ORCID iD orcid.org/0000-0002-1103-2484
ORCID for Sheila Francis: ORCID iD orcid.org/0000-0001-6552-0339
ORCID for Endre Kiss-Toth: ORCID iD orcid.org/0000-0003-4406-4017
ORCID for Andreas Schober: ORCID iD orcid.org/0000-0001-7798-136X
ORCID for Paul Hellewell: ORCID iD orcid.org/0000-0002-2044-1570
ORCID for Victoria Ridger: ORCID iD orcid.org/0000-0003-4163-669X

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Publisher:
Springer Science and Business Media LLC
Item Type:
Article
SWORD Depositor:
Symplectic Elements
Depositing User:
Symplectic Elements
Date record made live:
17 Feb 2026 15:17
Last Modified:
17 Feb 2026 15:30
Date of first compliant deposit:
5 February 2026
Date of first compliant Open Access:
17 February 2026
Version of first compliant deposit:
Version of Record
ISSN:
2041-1723
Volume:
11
URI:
https://shura.shu.ac.uk/id/eprint/36817
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