Multimodal imaging of metals in a retinal degeneration model to inform on ocular disease

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MILLAR, Joshua, GIBBONS, Luke, COSTA, Catia, SCHNEIDER, Ella, VON GERICHTEN, Johanna, BAILEY, Melanie J., CAMPBELL, Susan, DUCKETT, Catherine, DOYLE, Sarah and COLE, Laura (2023). Multimodal imaging of metals in a retinal degeneration model to inform on ocular disease. Analytica, 4 (3), 264-279. [Article]

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Abstract

The metallome has been involved in the pathological investigation into ocular tissue for decades; however, as technologies advance, more information can be ascertained from individual tissue sections that were not previously possible. Herein, a demonstration of complementary techniques has been utilized to describe the distribution and concentrations of essential metals in both wildtype (WT) and rhodopsin (Rho−/−) ocular tissues. The multimodal approach described is an example of complementary datasets that can be produced when employing a multifaceted analytical approach. Heterogenous distributions of copper and zinc were observable within both WT and Rho−/− tissue by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS), and the distributions of further trace elements notoriously problematic for ICP-MS analysis (phosphorous, Sulfur, chlorine, potassium, calcium, iron, and aluminum) were analysed by particle-induced X-ray emission (PIXE).

More Information

Official URL:

https://www.mdpi.com/2673-4532/4/3/21

Open Access URL:

https://www.mdpi.com/2673-4532/4/3/21/pdf?version=...

Open Access Version:

Published version

Additional Information:

** Article version: VoR ** From MDPI via Jisc Publications Router ** Licence for VoR version of this article: https://creativecommons.org/licenses/by/4.0/ ** Peer reviewed: TRUE ** Acknowledgements: Acknowledgments: We thank Rob Hutchinson (Elemental Scientific Lasers) for his expertise in working with novel equipment. For the purpose of open access, the author has applied a Creative Commons Attribution (CC BY) license to any author accepted manuscript version arising from this submission. **Journal IDs: eissn 2673-4532 **Article IDs: publisher-id: analytica-04-00021 **History: collection 09-2023; published_online 01-07-2023; accepted 24-06-2023; rev-recd 23-06-2023; submitted 31-05-2023

Uncontrolled Keywords:

laser ablation, age-related macular degeneration, multimodal, PIXE, ICP-MS, quantitative, ocular tissue, IBA, metallomics

Page Range:

264-279

Identifiers

Identification Number:

10.3390/analytica4030021

ORCID for Susan Campbell: