Epigenetic-mediated N -methyl-D-aspartate receptor changes in the brain of isolated reared rats

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LOUREIRO, Camila Marcelino, FACHIM, Helene Aparecida, CORSI-ZUELLI, Fabiana, SHUHAMA, Rosana, JOCA, Sâmia, MENEZES, Paulo Rossi, DALTON, Caroline, DEL-BEN, Cristina Marta, LOUZADA-JUNIOR, Paulo and REYNOLDS, Gavin P (2020). Epigenetic-mediated N -methyl-D-aspartate receptor changes in the brain of isolated reared rats. Epigenomics. [Article]

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27687:566336
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Abstract
Aim: We investigated: Grin1, Grin2a, Grin2b DNA methylation; NR1 and NR2 mRNA/protein in the prefrontal cortex (PFC); and hippocampus of male Wistar rats exposed to isolation rearing. Materials & methods: Animals were kept isolated or grouped (n = 10/group) from weaning for 10 weeks. Tissues were dissected for RNA/DNA extraction and N-methyl-D-aspartate receptor subunits were analyzed using quantitative reverse transcription (RT)-PCR, ELISA and pyrosequencing. Results: Isolated-reared animals had: decreased mRNA in PFC for all markers, increased NR1 protein in hippocampus and hypermethylation of Grin1 in PFC and Grin2b in hippocampus, compared with grouped rats. Associations between mRNA/protein and DNA methylation were found for both brain areas. Conclusion: This study indicates that epigenetic DNA methylation may underlie N-methyl-D-aspartate receptor mRNA/protein expression alterations caused by isolation rearing.
More Information
Official URL:
https://www.futuremedicine.com/doi/10.2217/epi-202...
Additional Information:
** Embargo end date: 26-11-2021 ** From Future Science Group via Jisc Publications Router **Journal IDs: pissn 1750-1911; eissn 1750-192X **History: published 26-11-2020; online 26-11-2020; accepted 18-09-2020; published 09-2020; submitted 16-04-2020 **License for this article: starting on 26-11-2021, , https://www.future-science-group.com/archiving
Uncontrolled Keywords:
Research Article, DNA methylation, early stress, gene expression, glutamate receptor, hippocampus, isolation rearing from weaning, NMDAR, prefrontal cortex, protein expression, schizophrenia

Identifiers

Identification Number:
10.2217/epi-2020-0151
ORCID for Camila Marcelino Loureiro: ORCID iD orcid.org/0000-0002-0239-5064
ORCID for Helene Aparecida Fachim: ORCID iD orcid.org/0000-0003-3818-3167
ORCID for Fabiana Corsi-Zuelli: ORCID iD orcid.org/0000-0001-9202-3987
ORCID for Rosana Shuhama: ORCID iD orcid.org/0000-0001-7626-7543
ORCID for Sâmia Joca: ORCID iD orcid.org/0000-0003-0255-5889
ORCID for Paulo Rossi Menezes: ORCID iD orcid.org/0000-0001-6330-3314
ORCID for Caroline Dalton: ORCID iD orcid.org/0000-0002-1404-873X
ORCID for Cristina Marta Del-Ben: ORCID iD orcid.org/0000-0003-0145-9975
ORCID for Paulo Louzada-Junior: ORCID iD orcid.org/0000-0003-2585-3870
ORCID for Gavin P Reynolds: ORCID iD orcid.org/0000-0001-9026-7726

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Item Type:
Article
SWORD Depositor:
Colin Knott
Depositing User:
Colin Knott
Date Deposited:
27 Nov 2020 10:57
Last Modified:
18 Dec 2024 20:48
Date of first compliant deposit:
27 January 2021
Date of first compliant Open Access:
26 November 2021
Version of first compliant deposit:
Author Accepted Manuscript
URI:
https://shura.shu.ac.uk/id/eprint/27687
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