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STOKES, Hannah, CROSS, Neil and LEYLAND, Rebecca (2018). Characterisation of novel lung cancer cell lines for immuno-inhibitory markers. In: BMRC/MERI Winter Poster Event 2018, Sheffield, 14 Dec 2018. BMRC/MERI. (Unpublished)
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BMRC poster FINAL.pdf - Accepted Version All rights reserved. Download (1MB) | Preview |
Abstract
The present study investigates the expression of immune biomarkers, PD-L1 and HLA-1 on novel lung cancer cell lines (H838, H838-EGFR, A549, A549-ALK, HCC 827, NCI 1650, TWIT, Jacket). PD-L1 and HLA-1 characterisation were initially performed and analysed via flow cytometry. These results showed that the expression of PD-L1 and HLA-1 varies across the cell lines from high percent to low. The effect of IFNy on biomarkers expression was also investigated following a 48 hour incubation period. of the cell lines analysed the expression of PD-L1 increases with IFNy stimulation whilst HLA-1 remains relatively unchanged. Trypan blue assays for cell viability were performed, showing that when stimulated, cells were 100% viable whereas viability decreases upon IFNy exposure.
| Item Type: | Conference or Workshop Item (Poster) |
|---|---|
| SWORD Depositor: | Symplectic Elements |
| Depositing User: | Symplectic Elements |
| Date Deposited: | 11 Feb 2019 14:02 |
| Last Modified: | 18 Mar 2021 05:21 |
| URI: | https://shura.shu.ac.uk/id/eprint/23639 |
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