Tools
Tools
DENG, Xiao, CAO, Shilin, LI, Ning, WU, Hong, SMITH, Thomas, ZONG, Minhua and LOU, Wenyong (2016). A magnetic biocatalyst based on mussel-inspired polydopamine and its acylation of dihydromyricetin. Chinese Journal of Catalysis, 37 (4), 584-595.
|
PDF
manuscript revision3.pdf - Accepted Version Creative Commons Attribution Non-commercial No Derivatives. Download (1MB) | Preview |
Abstract
A support made of mussel-inspired polydopamine-coated magnetic iron oxide nanoparticles (PD-MNPs) was prepared and characterized. The widely used Aspergillus niger lipase (ANL) was immobilized on the PD-MNPs (ANL@PD-MNPs) with a protein loading of 138 mg/g and an activity recovery of 83.6% under optimized conditions. For the immobilization, the pH and immobilization time were investigated. The pH and thermal and storage stability of the ANL@PD-MNPs significantly surpassed those of free ANL. The ANL@PD-MNPs had better solvent tolerance than free ANL. The secondary structure of free ANL and ANL@PD-MNPs was analyzed by infrared spectroscopy. A kinetic study demonstrated that the ANL@PD-MNPs had enhanced enzyme-substrate affinity and high catalytic efficiency. The ANL@PD-MNPs was applied as a biocatalyst for the regioselective acylation of dihydromyricetin (DMY) in DMSO and gave a conversion of 79.3%, which was higher than that of previous reports. The ANL@PD-MNPs retained over 55% of its initial activity after 10 cycles of reuse. The ANL@PD-MNPs were readily separated from the reaction system by a magnet. The PD-MNPs is an excellent support for ANL and the resulting ANL@PD-MNPs displayed good potential for the efficient synthesis of dihydromyricetin-3-acetate by enzymatic regioselective acylation.
| Item Type: | Article |
|---|---|
| Research Institute, Centre or Group - Does NOT include content added after October 2018: | Biomedical Research Centre |
| Identification Number: | https://doi.org/10.1016/S1872-2067(15)61045-2 |
| Page Range: | 584-595 |
| Depositing User: | Margaret Boot |
| Date Deposited: | 22 Aug 2016 11:27 |
| Last Modified: | 17 Mar 2021 15:31 |
| URI: | https://shura.shu.ac.uk/id/eprint/13289 |
Actions (login required)
 |
View Item |
Downloads
Downloads per month over past year