Polyurethane scaffold with in situ swelling capacity for nucleus pulposus replacement

LI, Zhen, LANG, Gernot, CHEN, Xu, SACKS, Hagit, MANTZUR, Carmit, TROPP, Udi, MADER, Kerstin T., SMALLWOOD, Thomas C., SAMMON, Chris, RICHARDS, R. Geoff, ALINI, Mauro and GRAD, Sibylle (2016). Polyurethane scaffold with in situ swelling capacity for nucleus pulposus replacement. Biomaterials, 84, 196 - 209.

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Abstract

Nucleus pulposus (NP) replacement offers a minimally invasive alternative to spinal fusion or total disc replacement for the treatment of intervertebral disc (IVD) degeneration. This study aimed to develop a cytocompatible {NP} replacement material, which is feasible for non-invasive delivery and tunable design, and allows immediate mechanical restoration of the IVD. A bi-phasic polyurethane scaffold was fabricated consisting of a core material with rapid swelling property and a flexible electrospun envelope. The scaffold was assessed in a bovine whole {IVD} organ culture model under dynamic load for 14 days. Nucleotomy was achieved by incision through the endplate without damaging the annulus fibrosus. After implantation of the scaffold and in situ swelling, the dynamic compressive stiffness and disc height were restored immediately. The scaffold also showed favorable cytocompatibility for native disc cells. Implantation of the scaffold in a partially nucleotomized {IVD} down-regulated catabolic gene expression, increased proteoglycan and type {II} collagen intensity and decreased type I collagen intensity in remaining {NP} tissue, indicating potential to retard degeneration and preserve the {IVD} cell phenotype. The scaffold can be delivered in a minimally invasive manner, and the geometry of the scaffold post-hydration is tunable by adjusting the core material, which allows individualized design. Keywords : Intervertebral disc degeneration

Item Type:	Article
Research Institute, Centre or Group - Does NOT include content added after October 2018:	Materials and Engineering Research Institute > Advanced Coatings and Composites Research Centre > Polymers, Composites and Spectroscopy Group
Identification Number:	https://doi.org/10.1016/j.biomaterials.2016.01.040
Page Range:	196 - 209
Depositing User:	Chris Sammon
Date Deposited:	11 Aug 2016 14:38
Last Modified:	18 Mar 2021 00:27
URI:	https://shura.shu.ac.uk/id/eprint/12665

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