Generation of a selective 5-HT3B subunit-recognising polyclonal antibody ; identification of immunoreactive cells in rat hippocampus.

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MONK, S A, DESAI, K, BRADY, C A, WILLIAMS, J M, LIN, L, PRINCIVALLE, A, HOPE, A G and BARNES, N M (2001). Generation of a selective 5-HT3B subunit-recognising polyclonal antibody ; identification of immunoreactive cells in rat hippocampus. Neuropharmacology, 41 (8), 1013-1016. [Article]

Abstract
The present study generated a polyclonal antibody (AP86/3) that recognises a peptide sequence of the h5-HT(3B) receptor subunit. Western blot analysis of homogenates prepared from cell lines expressing either homomeric (h5-HT(3A)) or heteromeric (h5-HT(3A/3B)) receptors, as well as immunocytochemical studies with the same cell lines, indicated that AP86/3 recognised, selectively, the 5-HT(3B) subunit. Immunohistochemical labelling was also apparent in cells in the rat hippocampus that displayed the distribution and morphology of interneurones.
More Information
Research Institute, Centre or Group - Does NOT include content added after October 2018:
Biomedical Research Centre
Page Range:
1013-1016

Identifiers

Identification Number:
10.1016/S0028-3908(01)00153-8
ORCID for A Princivalle: ORCID iD orcid.org/0000-0001-5375-0604

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Item Type:
Article
Depositing User:
Jamie Young
Date Deposited:
03 Jun 2015 08:57
Last Modified:
18 Mar 2021 23:15
URI:
https://shura.shu.ac.uk/id/eprint/9986
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