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MILLAR, Joshua, GIBBONS, Luke, COSTA, Catia, SCHNEIDER, Ella, VON GERICHTEN, Johanna, BAILEY, Melanie J., CAMPBELL, Susan, DUCKETT, Catherine, DOYLE, Sarah and COLE, Laura (2023). Multimodal imaging of metals in a retinal degeneration model to inform on ocular disease. Analytica, 4 (3), 264-279.
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Abstract
The metallome has been involved in the pathological investigation into ocular tissue for decades; however, as technologies advance, more information can be ascertained from individual tissue sections that were not previously possible. Herein, a demonstration of complementary techniques has been utilized to describe the distribution and concentrations of essential metals in both wildtype (WT) and rhodopsin (Rho−/−) ocular tissues. The multimodal approach described is an example of complementary datasets that can be produced when employing a multifaceted analytical approach. Heterogenous distributions of copper and zinc were observable within both WT and Rho−/− tissue by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS), and the distributions of further trace elements notoriously problematic for ICP-MS analysis (phosphorous, Sulfur, chlorine, potassium, calcium, iron, and aluminum) were analysed by particle-induced X-ray emission (PIXE).
| Item Type: | Article |
|---|---|
| Additional Information: | ** Article version: VoR ** From MDPI via Jisc Publications Router ** Licence for VoR version of this article: https://creativecommons.org/licenses/by/4.0/ ** Peer reviewed: TRUE ** Acknowledgements: Acknowledgments: We thank Rob Hutchinson (Elemental Scientific Lasers) for his expertise in working with novel equipment. For the purpose of open access, the author has applied a Creative Commons Attribution (CC BY) license to any author accepted manuscript version arising from this submission. **Journal IDs: eissn 2673-4532 **Article IDs: publisher-id: analytica-04-00021 **History: collection 09-2023; published_online 01-07-2023; accepted 24-06-2023; rev-recd 23-06-2023; submitted 31-05-2023 |
| Uncontrolled Keywords: | laser ablation, age-related macular degeneration, multimodal, PIXE, ICP-MS, quantitative, ocular tissue, IBA, metallomics |
| Identification Number: | https://doi.org/10.3390/analytica4030021 |
| Page Range: | 264-279 |
| SWORD Depositor: | Colin Knott |
| Depositing User: | Colin Knott |
| Date Deposited: | 11 Aug 2023 08:49 |
| Last Modified: | 11 Aug 2023 08:58 |
| URI: | https://shura.shu.ac.uk/id/eprint/32243 |
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