Multimodal imaging of metals in a retinal degeneration model to inform on ocular disease

MILLAR, Joshua, GIBBONS, Luke, COSTA, Catia, SCHNEIDER, Ella, VON GERICHTEN, Johanna, BAILEY, Melanie J., CAMPBELL, Susan, DUCKETT, Catherine, DOYLE, Sarah and COLE, Laura (2023). Multimodal imaging of metals in a retinal degeneration model to inform on ocular disease. Analytica, 4 (3), 264-279.

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The metallome has been involved in the pathological investigation into ocular tissue for decades; however, as technologies advance, more information can be ascertained from individual tissue sections that were not previously possible. Herein, a demonstration of complementary techniques has been utilized to describe the distribution and concentrations of essential metals in both wildtype (WT) and rhodopsin (Rho−/−) ocular tissues. The multimodal approach described is an example of complementary datasets that can be produced when employing a multifaceted analytical approach. Heterogenous distributions of copper and zinc were observable within both WT and Rho−/− tissue by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS), and the distributions of further trace elements notoriously problematic for ICP-MS analysis (phosphorous, Sulfur, chlorine, potassium, calcium, iron, and aluminum) were analysed by particle-induced X-ray emission (PIXE).

Item Type: Article
Additional Information: ** Article version: VoR ** From MDPI via Jisc Publications Router ** Licence for VoR version of this article: ** Peer reviewed: TRUE ** Acknowledgements: Acknowledgments: We thank Rob Hutchinson (Elemental Scientific Lasers) for his expertise in working with novel equipment. For the purpose of open access, the author has applied a Creative Commons Attribution (CC BY) license to any author accepted manuscript version arising from this submission. **Journal IDs: eissn 2673-4532 **Article IDs: publisher-id: analytica-04-00021 **History: collection 09-2023; published_online 01-07-2023; accepted 24-06-2023; rev-recd 23-06-2023; submitted 31-05-2023
Uncontrolled Keywords: laser ablation, age-related macular degeneration, multimodal, PIXE, ICP-MS, quantitative, ocular tissue, IBA, metallomics
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Page Range: 264-279
SWORD Depositor: Colin Knott
Depositing User: Colin Knott
Date Deposited: 11 Aug 2023 08:49
Last Modified: 11 Oct 2023 12:45

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