Epigenetic-mediated N -methyl-D-aspartate receptor changes in the brain of isolated reared rats

LOUREIRO, Camila Marcelino, FACHIM, Helene Aparecida, CORSI-ZUELLI, Fabiana, SHUHAMA, Rosana, JOCA, Sâmia, MENEZES, Paulo Rossi, DALTON, Caroline, DEL-BEN, Cristina Marta, LOUZADA-JUNIOR, Paulo and REYNOLDS, Gavin P (2020). Epigenetic-mediated N -methyl-D-aspartate receptor changes in the brain of isolated reared rats. Epigenomics.

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Link to published version:: https://doi.org/10.2217/epi-2020-0151


Aim: We investigated: Grin1, Grin2a, Grin2b DNA methylation; NR1 and NR2 mRNA/protein in the prefrontal cortex (PFC); and hippocampus of male Wistar rats exposed to isolation rearing. Materials & methods: Animals were kept isolated or grouped (n = 10/group) from weaning for 10 weeks. Tissues were dissected for RNA/DNA extraction and N-methyl-D-aspartate receptor subunits were analyzed using quantitative reverse transcription (RT)-PCR, ELISA and pyrosequencing. Results: Isolated-reared animals had: decreased mRNA in PFC for all markers, increased NR1 protein in hippocampus and hypermethylation of Grin1 in PFC and Grin2b in hippocampus, compared with grouped rats. Associations between mRNA/protein and DNA methylation were found for both brain areas. Conclusion: This study indicates that epigenetic DNA methylation may underlie N-methyl-D-aspartate receptor mRNA/protein expression alterations caused by isolation rearing.

Item Type: Article
Additional Information: ** Embargo end date: 26-11-2021 ** From Future Science Group via Jisc Publications Router **Journal IDs: pissn 1750-1911; eissn 1750-192X **History: published 26-11-2020; online 26-11-2020; accepted 18-09-2020; published 09-2020; submitted 16-04-2020 **License for this article: starting on 26-11-2021, , https://www.future-science-group.com/archiving
Uncontrolled Keywords: Research Article, DNA methylation, early stress, gene expression, glutamate receptor, hippocampus, isolation rearing from weaning, NMDAR, prefrontal cortex, protein expression, schizophrenia
Identification Number: https://doi.org/10.2217/epi-2020-0151
SWORD Depositor: Colin Knott
Depositing User: Colin Knott
Date Deposited: 27 Nov 2020 10:57
Last Modified: 26 Nov 2021 01:18
URI: https://shura.shu.ac.uk/id/eprint/27687

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