Generation of a selective 5-HT3B subunit-recognising polyclonal antibody ; identification of immunoreactive cells in rat hippocampus.

MONK, S A, DESAI, K, BRADY, C A, WILLIAMS, J M, LIN, L, PRINCIVALLE, A, HOPE, A G and BARNES, N M (2001). Generation of a selective 5-HT3B subunit-recognising polyclonal antibody ; identification of immunoreactive cells in rat hippocampus. Neuropharmacology, 41 (8), 1013-1016.

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Link to published version:: 10.1016/S0028-3908(01)00153-8

Abstract

The present study generated a polyclonal antibody (AP86/3) that recognises a peptide sequence of the h5-HT(3B) receptor subunit. Western blot analysis of homogenates prepared from cell lines expressing either homomeric (h5-HT(3A)) or heteromeric (h5-HT(3A/3B)) receptors, as well as immunocytochemical studies with the same cell lines, indicated that AP86/3 recognised, selectively, the 5-HT(3B) subunit. Immunohistochemical labelling was also apparent in cells in the rat hippocampus that displayed the distribution and morphology of interneurones.

Item Type: Article
Research Institute, Centre or Group: Biomolecular Sciences Research Centre
Identification Number: 10.1016/S0028-3908(01)00153-8
Depositing User: Jamie Young
Date Deposited: 03 Jun 2015 08:57
Last Modified: 03 Jun 2015 08:57
URI: http://shura.shu.ac.uk/id/eprint/9986

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