The division of amyloid fibrils – Systematic comparison of fibril fragmentation stability by linking theory with experiments

BEAL, David M, TOURNUS, Magali, MARCHANTE, Ricardo, PURTON, Tracey, SMITH, David, TUITE, Mick F, DOUMIC, Marie and XUE, Wei-Feng (2020). The division of amyloid fibrils – Systematic comparison of fibril fragmentation stability by linking theory with experiments. iScience, p. 101512.

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Official URL: https://www.sciencedirect.com/science/article/pii/...
Open Access URL: https://www.sciencedirect.com/science/article/pii/... (Published)
Link to published version:: https://doi.org/10.1016/j.isci.2020.101512
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    Abstract

    The division of amyloid protein fibrils is required for the propagation of the amyloid state, and is an important contributor to their stability, pathogenicity and normal function. Here, we combine kinetic nanoscale imaging experiments with analysis of a mathematical model to resolve and compare the division stability of amyloid fibrils. Our theoretical results show that the division of any type of filament results in self-similar length distributions distinct to each fibril type and the conditions applied. By applying these theoretical results to profile the dynamical stability towards breakage for four different amyloid types, we reveal particular differences in the division properties of disease-related amyloid formed from alpha-synuclein when compared with non-disease associated model amyloid, the former showing lowered intrinsic stability towards breakage and increased likelihood of shedding smaller particles. Our results enable the comparison of protein filaments’ intrinsic dynamic stabilities, which are key to unravelling their toxic and infectious potentials.

    Item Type: Article
    Identification Number: https://doi.org/10.1016/j.isci.2020.101512
    Page Range: p. 101512
    SWORD Depositor: Symplectic Elements
    Depositing User: Symplectic Elements
    Date Deposited: 09 Sep 2020 12:23
    Last Modified: 09 Sep 2020 12:23
    URI: http://shura.shu.ac.uk/id/eprint/27183

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