Study of the stability of functionalized gold nanoparticles for the colorimetric detection of dipeptidyl peptidase IV

ALDEWACHI, Hasan, WOODROOFE, Nicola and GARDINER, Philip (2018). Study of the stability of functionalized gold nanoparticles for the colorimetric detection of dipeptidyl peptidase IV. Applied Sciences, 8 (12), p. 2589.

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Official URL: https://www.mdpi.com/2076-3417/8/12/2589
Open Access URL: https://www.mdpi.com/2076-3417/8/12/2589 (Published)
Link to published version:: https://doi.org/10.3390/app8122589
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    Abstract

    In this report, we investigated three stabilization strategies of gold nanoparticles and their practical application for the visual detection of dipeptidyl peptidase IV (DPP-IV). Citrate-capped gold nanoparticles (Au NPs) are generally unstable in high-ionic-strength samples. Au NPs are easily tagged with various proteins and biomolecules rich in amino acids, leading to important biomedical applications including targeted drug delivery, cellular imaging, and biosensing. The investigated assays were based on different modes of stabilization, such as the incorporation of polyethylene glycol (PEG) groups, stabilizer peptide, and bifunctionalization. Although all approaches provided highly stable Au NP platforms demonstrated by zeta potential measurements and resistance to aggregation in a high-ionic-strength saline solution, we found that the Au NPs modified with a separate stabilizer ligand provided the highest stability and was the only platform that demonstrated sensitivity to the addition of DPP-IV, whilst PEGylated and peptide-stabilized Au NPs showed no significant response.

    Item Type: Article
    Uncontrolled Keywords: gold nanoparticles; stabilization strategies; colorimetric detection; dipeptidyl peptidase IV
    Identification Number: https://doi.org/10.3390/app8122589
    Page Range: p. 2589
    SWORD Depositor: Symplectic Elements
    Depositing User: Symplectic Elements
    Date Deposited: 10 Dec 2019 10:35
    Last Modified: 10 Dec 2019 10:45
    URI: http://shura.shu.ac.uk/id/eprint/25456

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