Biomineralisation performance of bacteria isolated from a landfill in China

RAJASEKAR, Adharsh, WILKINSON, Stephen, SEKAR, Raju, BRIDGE, Jonathan, MEDINA-ROLDAN, Eduardo and MOY, Charles K.S (2018). Biomineralisation performance of bacteria isolated from a landfill in China. Canadian Journal of Microbiology (CJM), 64 (12), 945-953.

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Link to published version:: https://doi.org/10.1139/cjm-2018-0254

Abstract

We report an investigation of microbially-induced carbonate precipitation by seven indigenous bacteria isolated from a landfill in China. Bacterial strains were cultured in a medium supplemented with 25 mM calcium chloride and 333 mM urea. The experiments were carried out at 30 °C for 7 days with agitation by a shaking table at 130 rpm. Scanning Electron Microscopic (SEM) and X-ray diffraction (XRD) analyses showed variations in calcium carbonate polymorphs and mineral composition induced by all bacterial strains. The amount of carbonate precipitation was quantified by titration. The amount of carbonate precipitated in the medium varied among isolates with the lowest being Bacillus aerius rawirorabr15 (LC092833) precipitating around 1.5 times more carbonate per unit volume than the abiotic (blank) solution. Pseudomonas nitroreducens szh_asesj15 (LC090854) was found to be the most efficient, precipitating 3.2 times more carbonate than the abiotic solution. Our results indicate that bacterial carbonate precipitation occurred through ureolysis and suggest that variations in carbonate crystal polymorphs and rates of precipitation were driven by strain-specific differences in urease expression and response to the alkaline environment. These results and the method applied provide benchmarking/screening data for assessing the bioremediation potential of indigenous bacteria for containment of contaminants in landfills.

Item Type: Article
Additional Information: ** From Crossref via Jisc Publications Router. **Journal IDs: pissn 0008-4166; eissn 1480-3275
Uncontrolled Keywords: Immunology, Applied Microbiology and Biotechnology, Genetics, Molecular Biology, Microbiology, General Medicine
Departments - Does NOT include content added after October 2018: Faculty of Social Sciences and Humanities > Department of Natural and Build Environment
Identification Number: https://doi.org/10.1139/cjm-2018-0254
SWORD Depositor: Margaret Boot
Depositing User: Margaret Boot
Date Deposited: 10 Sep 2018 14:57
Last Modified: 05 Dec 2018 13:30
URI: http://shura.shu.ac.uk/id/eprint/22438

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