Thermally Triggered Hydrogel Injection Into Bovine Intervertebral Disc Tissue Explants Induces Differentiation Of Mesenchymal Stem Cells And Restores Mechanical Function.

THORPE, Abbey, DOUGILL, G, VICKERS, Louise, REEVES, N, COOPER, G, SAMMON, Chris and LE MAITRE, Christine (2017). Thermally Triggered Hydrogel Injection Into Bovine Intervertebral Disc Tissue Explants Induces Differentiation Of Mesenchymal Stem Cells And Restores Mechanical Function. Acta Biomaterialia, 54, 212-226.

[img]
Preview
PDF
Thorpe thermally triggered hydrogel.pdf - Accepted Version
All rights reserved.

Download (488kB) | Preview
[img]
Preview
Image (JPEG)
Figure 1 Identification of Injection Site.jpg - Supplemental Material
All rights reserved.

Download (145kB) | Preview
[img]
Preview
Image (JPEG)
Figure 2 Caspase 3 IHC.jpg - Supplemental Material
All rights reserved.

Download (98kB) | Preview
[img]
Preview
Image (JPEG)
Figure 3 proof change.jpg - Supplemental Material
All rights reserved.

Download (92kB) | Preview
[img]
Preview
Image (JPEG)
Figure 4 Mechanical analysis.jpg - Supplemental Material

Download (81kB) | Preview
[img]
Preview
Image (JPEG)
Figure 5 Histology Matrix Deposition.jpg - Supplemental Material
All rights reserved.

Download (226kB) | Preview
[img]
Preview
Image (JPEG)
Figure 6 IHC.jpg - Supplemental Material
All rights reserved.

Download (155kB) | Preview
[img] Microsoft Word
Supplementary Fig 1.docx - Supplemental Material
All rights reserved.

Download (54kB)
Official URL: https://www.sciencedirect.com/science/article/pii/...
Link to published version:: https://doi.org/10.1016/j.actbio.2017.03.010

Abstract

We previously reported a synthetic Laponite® crosslinked pNIPAM-co-DMAc (L-pNIPAM-co-DMAc) hydrogel which promotes differentiation of mesenchymal stem cells (MSCs) to nucleus pulposus (NP) cells without additional growth factors. The clinical success of this hydrogel is dependent on: integration with surrounding tissue; the capacity to restore mechanical function; as well as supporting the viability and differentiation of delivered MSCs. Bovine NP tissue explants were injected with media (control), human MSCs (hMSCs) alone, acellular L-pNIPAM-co-DMAc hydrogel or hMSCs incorporated within the L-pNIPAM-co-DMAc hydrogel and maintained at 5% O2 for 6 weeks. Viability of native NP cells and delivered MSCs was maintained. Furthermore hMSCs delivered via the L-pNIPAM-co-DMAc hydrogel differentiated and produced NP matrix components: aggrecan, collagen type II and chondroitin sulphate, with integration of the hydrogel with native NP tissue. In addition L-pNIPAM-co-DMAc hydrogel injected into collagenase digested bovine discs filled micro and macro fissures, were maintained within the disc during loading and restored IVD stiffness. The mechanical support of the L-pNIPAM-co-DMAc hydrogel, to restore disc height, could provide immediate symptomatic pain relief, whilst the delivery of MSCs over time regenerates the NP extracellular matrix; thus the L-pNIPAM-co-DMAc hydrogel could provide a combined cellular and mechanical repair approach.

Item Type: Article
Research Institute, Centre or Group - Does NOT include content added after October 2018: Biomedical Research Centre
Identification Number: https://doi.org/10.1016/j.actbio.2017.03.010
Page Range: 212-226
Depositing User: Users 3084 not found.
Date Deposited: 11 Apr 2017 15:43
Last Modified: 17 Mar 2021 20:45
URI: https://shura.shu.ac.uk/id/eprint/15537

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics