Registration of T-2 mycotoxin with total internal reflection ellipsometry and QCM impedance methods

NABOK, A. V., TSARGORODSKAYA, A., HOLLOWAY, A., STARODUB, N. F. and GOJSTER, O. (2007). Registration of T-2 mycotoxin with total internal reflection ellipsometry and QCM impedance methods. Biochemical and Biomolecular Sensing, 22 (6), 885-890.

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Abstract

A sensitive optical method of total internal reflection ellipsometry (TIRE) in conjunction with immune assay approach was exploited for the registration of T-2 mycotoxin in a wide range of concentrations from 100 mu g/mI down to 0.15 ng/ml. Association constants of 1.4 x 10(6) and 1.9 x 10(7) mol(-1) s for poly- and monoclonal T-2 antibodies, respectively, were evaluated from TIRE kinetic measurements. According to TIRE data fitting, binding of T-2 molecules to antibodies (at saturation) has resulted in the increase in adsorbed layer thickness of 4-5 nm. The QCM impedance measurements data showed anomalously large mass increase and film softening, most likely, due to the binding of large T-2 aggregates to antibodies. (c) 2006 Elsevier B.V. All rights reserved.

Item Type:	Article
Research Institute, Centre or Group - Does NOT include content added after October 2018:	Materials and Engineering Research Institute > Advanced Coatings and Composites Research Centre > Electronic Materials and Sensors Research Group
Identification Number:	https://doi.org/10.1016/j.bios.2006.03.010
Page Range:	885-890
Depositing User:	Ann Betterton
Date Deposited:	01 Apr 2010 14:47
Last Modified:	18 Mar 2021 21:30
URI:	https://shura.shu.ac.uk/id/eprint/1433

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