A magnetic biocatalyst based on mussel-inspired polydopamine and its acylation of dihydromyricetin

DENG, Xiao, CAO, Shilin, LI, Ning, WU, Hong, SMITH, Thomas, ZONG, Minhua and LOU, Wenyong (2016). A magnetic biocatalyst based on mussel-inspired polydopamine and its acylation of dihydromyricetin. Chinese Journal of Catalysis, 37 (4), 584-595.

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Official URL: http://www.cjcatal.org/EN/abstract/abstract21824.s...
Link to published version:: 10.1016/S1872-2067(15)61045-2


A support made of mussel-inspired polydopamine-coated magnetic iron oxide nanoparticles (PD-MNPs) was prepared and characterized. The widely used Aspergillus niger lipase (ANL) was immobilized on the PD-MNPs (ANL@PD-MNPs) with a protein loading of 138 mg/g and an activity recovery of 83.6% under optimized conditions. For the immobilization, the pH and immobilization time were investigated. The pH and thermal and storage stability of the ANL@PD-MNPs significantly surpassed those of free ANL. The ANL@PD-MNPs had better solvent tolerance than free ANL. The secondary structure of free ANL and ANL@PD-MNPs was analyzed by infrared spectroscopy. A kinetic study demonstrated that the ANL@PD-MNPs had enhanced enzyme-substrate affinity and high catalytic efficiency. The ANL@PD-MNPs was applied as a biocatalyst for the regioselective acylation of dihydromyricetin (DMY) in DMSO and gave a conversion of 79.3%, which was higher than that of previous reports. The ANL@PD-MNPs retained over 55% of its initial activity after 10 cycles of reuse. The ANL@PD-MNPs were readily separated from the reaction system by a magnet. The PD-MNPs is an excellent support for ANL and the resulting ANL@PD-MNPs displayed good potential for the efficient synthesis of dihydromyricetin-3-acetate by enzymatic regioselective acylation.

Item Type: Article
Research Institute, Centre or Group: Biomolecular Sciences Research Centre
Identification Number: 10.1016/S1872-2067(15)61045-2
Depositing User: Margaret Boot
Date Deposited: 22 Aug 2016 11:27
Last Modified: 22 Aug 2016 11:30
URI: http://shura.shu.ac.uk/id/eprint/13289

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