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LUI, Jennifer, CASTELLI, Lydia M., PIZZINGA, Mariavittoria, SIMPSON, Clare E., HOYLE, Nathaniel P., BAILEY, Kathryn L., CAMPBELL, Susan G. and ASHE, Mark P. (2014). Granules harboring translationally active mRNAs provide a platform for P-Body formation following stress. Cell Reports, 9 (3), 944-954.
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Abstract
The localization of mRNA to defined cytoplasmic sites in eukaryotic cells not only allows localized protein production but also determines the fate of mRNAs. For instance, translationally repressed mRNAs localize to P-bodies and stress granules where their decay and storage, respectively, are directed. Here, we find that several mRNAs are localized to granules in unstressed, actively growing cells. These granules play a key role in the stress-dependent formation of P-bodies. Specific glycolytic mRNAs are colocalized in multiple granules per cell, which aggregate during P-body formation. Such aggregation is still observed under conditions or in mutants where P-bodies do not form. In unstressed cells, the mRNA granules appear associated with active translation; this might enable a coregulation of protein expression from the same pathways or complexes. Parallels can be drawn between this coregulation and the advantage of operons in prokaryotic systems.
| Item Type: | Article |
|---|---|
| Research Institute, Centre or Group - Does NOT include content added after October 2018: | Biomedical Research Centre |
| Identification Number: | https://doi.org/10.1016/j.celrep.2014.09.040 |
| Page Range: | 944-954 |
| Depositing User: | Louise Vickers |
| Date Deposited: | 05 Nov 2014 12:22 |
| Last Modified: | 17 Mar 2021 20:16 |
| URI: | https://shura.shu.ac.uk/id/eprint/8585 |
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