Multipotential electrochemical detection of primer extension reactions on DNA self-assembled monolayers

DI GIUSTO, D. A., WLASSOFF, W. A., GIESEBRECHT, S., GOODING, J. J. and KING, G. C. (2003). Multipotential electrochemical detection of primer extension reactions on DNA self-assembled monolayers. Journal of the American Chemical Society, 126 (13), 4120-4121. [Article]

Abstract

Electroactive nucleoside triphosphates ("electrotides") have been incorporated into primers by DNA polymerase and detected on oligonucleotide surface-assembled monolayers. Four electrotides bearing three different electroactive moieties-ferrocene, vinylferrocene, and anthraquinone are detected in four alternative formats.

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