Signal recognition particle mediates post-translational targeting in eukaryotes

ABELL, Benjamin, POOL, Martin R., SCHLENKER, Oliver, SINNING, Irmgard and HIGH, Stephen (2004). Signal recognition particle mediates post-translational targeting in eukaryotes. The EMBO Journal, 23 (14), 2755-2764.

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Signal recognition particle (SRP) plays a central role in the delivery of classical secretory and membrane proteins to the endoplasmic reticulum (ER). All nascent chains studied to date dissociate from SRP once released from the ribosome, thereby supporting a strictly cotranslational mode of action for eukaryotic SRP. We now report a novel post-translational function for SRP in the targeting of tail-anchored (TA) proteins to the ER. TA proteins possess a hydrophobic membrane insertion sequence at their C-terminus such that it can only emerge from the ribosome after translation is terminated. We show that SRP can associate post-translationally with this type of ER-targeting signal, and deliver newly synthesised TA proteins to the ER membrane by a pathway dependent upon GTP and the SRP receptor. We find that dependency upon this SRP-dependent route is precursor specific, and propose a unifying model to describe the biogenesis of TA proteins in vivo.

Item Type: Article
Uncontrolled Keywords: ER targeting, membrane protein biogenesis, SRP, tail-anchored protein
Research Institute, Centre or Group - Does NOT include content added after October 2018: Biomedical Research Centre
Identification Number:
Page Range: 2755-2764
Depositing User: Ann Betterton
Date Deposited: 15 Feb 2008
Last Modified: 18 Mar 2021 21:45

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