Jasmonate biosynthesis regulates key events in barley vegetative and reproductive development

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LUO, Jian, LIU, Linsan, YANG, Xiujuan, DOOLAN, Olivia, DUANGTA, Tanicha, LYON, Emily, GRIFFIN, Jonathan, SHOESMITH, Jennifer, WU, Wenhao, HAMILTON, Ruth, NUTER, Mirjam, KATAY-FODOR, Mikah, BRICKLEBANK, Neil, ABERDEIN, Nicola, ZHANG, Guoping, TURNBULL, Colin, TUCKER, Matthew and MCKIM, Sarah M (2026). Jasmonate biosynthesis regulates key events in barley vegetative and reproductive development. New Phytologist. [Article]

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Abstract
Cereal grain is a global food staple. Filial tissues in grain, such as the embryo and endosperm, grow embedded in the maternal ovule and surrounding ovary wall. Maternal tissues provide nutrition and protection but are progressively reduced and degraded as filial tissues expand, driving grain expansion between floret hulls. While we know some enzymatic players, regulation of this process in temperate cereals like wheat and barley remains less understood. However, our previous work suggested a role for the phytohormone Jasmonate (JA). To learn more, we edited the barley ALLENE OXIDASE CYCLASE (HvAOC) gene encoding a JA biosynthetic enzyme. Defective HvAOC alleles reduced JA levels, and caused enhanced vegetative growth, faster flowering and enlarged grain - phenotypes rescued by MeJA treatment. HvAOC control appears parentally-derived, consistent with enriched HvAOC expression in maternal compared to filial tissues. Grain dimension changes were uncoupled from hull size, and correlated with larger and more persistent maternal tissues, increased endosperm, and higher expression of genes linked to sucrose transport. This work provides the first genetic dissection of JA in barley and highlights a key role in the coordination between growth and death of maternal tissues within grain.
More Information
Uncontrolled Keywords:
Jasmonate; Allene Oxidase Cyclase; Barley; Grain; Development; Gene editing; 06 Biological Sciences; 07 Agricultural and Veterinary Sciences; Plant Biology & Botany; 3108 Plant biology; 4101 Climate change impacts and adaptation; 4102 Ecological applications

Identifiers

ORCID for Neil Bricklebank: ORCID iD orcid.org/0000-0002-1614-2260
ORCID for Nicola Aberdein: ORCID iD orcid.org/0000-0002-6147-4221

Library

Publisher:
Wiley
Item Type:
Article
SWORD Depositor:
Symplectic Elements
Depositing User:
Symplectic Elements
Date record made live:
13 Aug 2026 13:59
Last Modified:
13 Aug 2026 14:00
Date of first compliant deposit:
13 August 2026
Version of first compliant deposit:
Author Accepted Manuscript
ISSN:
0028-646X
URI:
https://shura.shu.ac.uk/id/eprint/37833
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