The dynamic architecture of the metabolic switch in Streptomyces coelicolor.

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NIESELT, Kay, BATTKE, Florian, HERBIG, Alexander, BRUHEIM, Per, WENTZEL, Alexander, JAKOBSEN, Øyvind M, SLETTA, Håvard, ALAM, Mohammad T, MERLO, Maria E, MOORE, Jonathan, OMARA, Walid, MORRISSEY, Edward R, JUAREZ-HERMOSILLO, Miguel A, RODRÍGUEZ-GARCÍA, Antonio, NENTWICH, Merle, THOMAS, Louise, IQBAL, Mudassar, LEGAIE, Roxane, GAZE, William H, CHALLIS, Gregory L, JANSEN, Ritsert C, DIJKHUIZEN, Lubbert, RAND, David A, WILD, David L, BONIN, Michael, REUTHER, Jens, WOHLLEBEN, Wolfgang, SMITH, Margaret CM, BURROUGHS, Nigel J, MARTÍN, Juan F, HODGSON, David A, TAKANO, Eriko, BREITLING, Rainer, ELLINGSEN, Trond E and WELLINGTON, Elizabeth MH (2010). The dynamic architecture of the metabolic switch in Streptomyces coelicolor. BMC genomics, 11 (1): 10. [Article]

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Abstract

Background

During the lifetime of a fermenter culture, the soil bacterium S. coelicolor undergoes a major metabolic switch from exponential growth to antibiotic production. We have studied gene expression patterns during this switch, using a specifically designed Affymetrix genechip and a high-resolution time-series of fermenter-grown samples.

Results

Surprisingly, we find that the metabolic switch actually consists of multiple finely orchestrated switching events. Strongly coherent clusters of genes show drastic changes in gene expression already many hours before the classically defined transition phase where the switch from primary to secondary metabolism was expected. The main switch in gene expression takes only 2 hours, and changes in antibiotic biosynthesis genes are delayed relative to the metabolic rearrangements. Furthermore, global variation in morphogenesis genes indicates an involvement of cell differentiation pathways in the decision phase leading up to the commitment to antibiotic biosynthesis.

Conclusions

Our study provides the first detailed insights into the complex sequence of early regulatory events during and preceding the major metabolic switch in S. coelicolor, which will form the starting point for future attempts at engineering antibiotic production in a biotechnological setting.
More Information
Official URL:
https://doi.org/10.1186/1471-2164-11-10
Uncontrolled Keywords:
Streptomyces coelicolor; RNA, Bacterial; Anti-Bacterial Agents; Cluster Analysis; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Fermentation; Genes, Bacterial; Multigene Family; Anti-Bacterial Agents; Cluster Analysis; Fermentation; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Genes, Bacterial; Multigene Family; RNA, Bacterial; Streptomyces coelicolor; 06 Biological Sciences; 08 Information and Computing Sciences; 11 Medical and Health Sciences; Bioinformatics; 31 Biological sciences; 32 Biomedical and clinical sciences
Event Location:
England

Identifiers

Identification Number:
10.1186/1471-2164-11-10
ORCID for Florian Battke: ORCID iD orcid.org/0000-0001-6021-5837
ORCID for Alexander Herbig: ORCID iD orcid.org/0000-0003-1176-1166
ORCID for Per Bruheim: ORCID iD orcid.org/0000-0002-7851-8083
ORCID for Alexander Wentzel: ORCID iD orcid.org/0000-0002-9420-1959
ORCID for Håvard Sletta: ORCID iD orcid.org/0000-0002-5635-5674
ORCID for Mohammad T Alam: ORCID iD orcid.org/0000-0002-6872-0691
ORCID for Maria E Merlo: ORCID iD orcid.org/0000-0003-2208-9467
ORCID for Jonathan Moore: ORCID iD orcid.org/0000-0002-5486-0407
ORCID for Walid Omara: ORCID iD orcid.org/0000-0001-5086-8260
ORCID for Edward R Morrissey: ORCID iD orcid.org/0000-0003-1021-5358
ORCID for Antonio Rodríguez-García: ORCID iD orcid.org/0000-0001-8118-9762
ORCID for Mudassar Iqbal: ORCID iD orcid.org/0000-0002-5006-4331
ORCID for William H Gaze: ORCID iD orcid.org/0000-0002-9345-6204
ORCID for Gregory L Challis: ORCID iD orcid.org/0000-0001-5976-3545
ORCID for Lubbert Dijkhuizen: ORCID iD orcid.org/0000-0003-2312-7162
ORCID for David A Rand: ORCID iD orcid.org/0000-0002-2217-3274
ORCID for Margaret CM Smith: ORCID iD orcid.org/0000-0002-4150-0496
ORCID for Nigel J Burroughs: ORCID iD orcid.org/0000-0002-4632-1550
ORCID for Eriko Takano: ORCID iD orcid.org/0000-0002-6791-3256
ORCID for Rainer Breitling: ORCID iD orcid.org/0000-0001-7173-0922
ORCID for Elizabeth MH Wellington: ORCID iD orcid.org/0000-0002-4329-0699

Library

Item Type:
Article
SWORD Depositor:
Symplectic Elements
Depositing User:
Symplectic Elements
Date record made live:
15 Jan 2026 17:34
Last Modified:
15 Jan 2026 17:45
Date of first compliant deposit:
8 November 2025
Date of first compliant Open Access:
15 January 2026
Version of first compliant deposit:
Version of Record
URI:
https://shura.shu.ac.uk/id/eprint/36370
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