Enhanced Expression of Autoantigens During SARS-CoV-2 Viral Infection

SAHEB SHARIF-ASKARI, Narjes, SAHEB SHARIF-ASKARI, Fatemeh, AHMED, Samrein, HANNAWI, Suad, HAMOUDI, Rifat, HAMID, Qutayba and HALWANI, Rabih (2021). Enhanced Expression of Autoantigens During SARS-CoV-2 Viral Infection. Frontiers in Immunology, 12: 686462.

[img]
Preview
PDF
fimmu-12-686462.pdf - Published Version
Creative Commons Attribution.

Download (3MB) | Preview
Official URL: https://www.frontiersin.org/articles/10.3389/fimmu...
Link to published version:: https://doi.org/10.3389/fimmu.2021.686462

Abstract

Immune homeostasis is disturbed during severe viral infections, which can lead to loss of tolerance to self-peptides and result in short- or long-term autoimmunity. Using publicly available transcriptomic datasets, we conducted an in-silico analyses to evaluate the expression levels of 52 autoantigens, known to be associated with 24 autoimmune diseases, during SAR-CoV-2 infection. Seven autoantigens (MPO, PRTN3, PADI4, IFIH1, TRIM21, PTPRN2, and TSHR) were upregulated in whole blood samples. MPO and TSHR were overexpressed in both lung autopsies and whole blood tissue and were associated with more severe COVID-19. Neutrophil activation derived autoantigens (MPO, PRTN3, and PADI4) were prominently increased in blood of both SARS-CoV-1 and SARS-CoV-2 viral infections, while TSHR and PTPRN2 autoantigens were specifically increased in SARS-CoV-2. Using single-cell dataset from peripheral blood mononuclear cells (PBMCs), we observed an upregulation of MPO, PRTN3, and PADI4 autoantigens within the low-density neutrophil subset. To validate our in-silico analysis, we measured plasma protein levels of two autoantigens, MPO and PRTN3, in severe and asymptomatic COVID-19. The protein levels of these two autoantigens were significantly upregulated in more severe COVID-19 infections. In conclusion, the immunopathology and severity of COVID-19 could result in transient autoimmune activation. Longitudinal follow-up studies of confirmed cases of COVID-19 could determine the enduring effects of viral infection including development of autoimmune disease.

Item Type: Article
Uncontrolled Keywords: 1107 Immunology; 1108 Medical Microbiology; 3101 Biochemistry and cell biology; 3105 Genetics; 3204 Immunology
Identification Number: https://doi.org/10.3389/fimmu.2021.686462
SWORD Depositor: Symplectic Elements
Depositing User: Symplectic Elements
Date Deposited: 25 Jan 2024 15:37
Last Modified: 25 Jan 2024 15:45
URI: https://shura.shu.ac.uk/id/eprint/33034

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics