Cellular Responses to Extracellular Vesicles as Potential Markers of Colorectal Cancer Progression

GUARNERIO, Sonia, TEMPEST, Robert, MAANI, Rawan, HUNT, Stuart, COLE, Laura M., LE MAITRE, Christine L., CHAPPLE, Keith and PEAKE, Nicholas (2023). Cellular Responses to Extracellular Vesicles as Potential Markers of Colorectal Cancer Progression. International Journal of Molecular Sciences, 24 (23): 16755.

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Official URL: https://www.mdpi.com/1422-0067/24/23/16755
Open Access URL: https://www.mdpi.com/1422-0067/24/23/16755/pdf?ver... (Published version)
Link to published version:: https://doi.org/10.3390/ijms242316755


The development of novel screening tests aims to support early asymptomatic diagnosis and subtyping patients according to similar traits in the heterogeneous cancer cohort. Extracellular vesicles (EVs) are promising candidates for the detection of disease markers from bodily fluids, but limitations in the standardisation of isolation methods and the intrinsic EV heterogeneity obtained from liquid biopsies are currently obstacles to clinical adoption. Here, cellular responses to cancer EVs were initially explored as potential complementary biomarkers for stage separation using colorectal cancer (CRC) SW480 and SW620 cell line models. A pilot study on a small cohort of CRC patients and controls was then developed by performing a multivariate analysis of cellular responses to plasma-derived EVs. Several cell activities and markers involved in tumour microenvironment pathways were influenced by the treatment of cell line EVs in a stage-dependent manner. The multivariate analysis combining plasma EV markers and cellular responses to plasma EVs was able to separate patients according to disease stage. This preliminary study offers the potential of considering cellular responses to EVs in combination with EV biomarkers in the development of screening methods.

Item Type: Article
Additional Information: ** Article version: VoR ** From MDPI via Jisc Publications Router ** Licence for VoR version of this article: https://creativecommons.org/licenses/by/4.0/ ** Peer reviewed: TRUE ** Acknowledgements: We kindly acknowledge Chris Hill at the University of Sheffield Electron Microscopy Facility, Faculty of Science, University of Sheffield, for the excellent support in obtaining TEM images. We also acknowledge the full clinical research team at Sheffield Teaching Hospitals who supported collection of blood from CRC patients, and Nicola Tattersall of the Technical Operations Resources and Services at Sheffield Hallam University for supporting blood collection from volunteers, as well as all staff and students who volunteered samples for this study. **Journal IDs: eissn 1422-0067 **Article IDs: publisher-id: ijms-24-16755 **History: collection 12-2023; published_online 25-11-2023; accepted 22-11-2023; rev-recd 13-11-2023; submitted 13-10-2023
Uncontrolled Keywords: extracellular vesicles, responsive biomarkers, colorectal cancer
Identification Number: https://doi.org/10.3390/ijms242316755
SWORD Depositor: Colin Knott
Depositing User: Colin Knott
Date Deposited: 07 Dec 2023 17:08
Last Modified: 07 Dec 2023 17:15
URI: https://shura.shu.ac.uk/id/eprint/32840

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