Recommendations for intervertebral disc notochordal cell investigation: From isolation to characterization

WILLIAMS, Rebecca J., LAAGLAND, Lisanne T., BACH, Frances C., WARD, Lizzy, CHAN, Wilson, TAM, Vivian, MEDZIKOVIC, Adel, BASATVAT, Shaghayegh, PAILLAT, Lily, VEDRENNE, Nicolas, SNUGGS, Joseph W., PORAMBA‐LIYANAGE, Deepani W., HOYLAND, Judith A., CHAN, Danny, CAMUS, Anne, RICHARDSON, Stephen M., TRYFONIDOU, Marianna A. and LE MAITRE, Christine L. (2023). Recommendations for intervertebral disc notochordal cell investigation: From isolation to characterization. JOR Spine: e1272.

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Abstract

Background: Lineage‐tracing experiments have established that the central region of the mature intervertebral disc, the nucleus pulposus (NP), develops from the embryonic structure called “the notochord”. However, changes in the cells derived from the notochord which form the NP (i.e., notochordal cells [NCs]), in terms of their phenotype and functional identity from early developmental stages to skeletal maturation are less understood. These key issues require further investigation to better comprehend the role of NCs in homeostasis and degeneration as well as their potential for regeneration. Progress in utilizing NCs is currently hampered due to poor consistency and lack of consensus methodology for in vitro NC extraction, manipulation, and characterization. Methods: Here, an international group has come together to provide key recommendations and methodologies for NC isolation within key species, numeration, in vitro manipulation and culture, and characterization. Results: Recommeded protocols are provided for isolation and culture of NCs. Experimental testing provided recommended methodology for numeration of NCs. The issues of cryopreservation are demonstrated, and a pannel of immunohistochemical markers are provided to inform NC characterization. Conclusions: Together we hope this article provides a road map for in vitro studies of NCs to support advances in research into NC physiology and their potential in regenerative therapies.

Item Type:	Article
Additional Information:	** Article version: VoR ** From Wiley via Jisc Publications Router ** Licence for VoR version of this article: http://creativecommons.org/licenses/by-nc/4.0/ **Journal IDs: issn 2572-1143 **Article IDs: publisher-id: jsp21272 **History: published_online 09-07-2023; accepted 21-06-2023; rev-recd 16-06-2023; submitted 14-04-2023
Uncontrolled Keywords:	culture systems, nucleus pulposus, notochordal cells, development, intervertebral disc, tissue‐specific progenitor cells
Identification Number:	https://doi.org/10.1002/jsp2.1272
SWORD Depositor:	Colin Knott
Depositing User:	Colin Knott
Date Deposited:	13 Jul 2023 10:06
Last Modified:	13 Jul 2023 10:06
URI:	https://shura.shu.ac.uk/id/eprint/32113

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