Self-association of the glycopeptide antibiotic teicoplanin A2 in aqueous solution studied by molecular hydrodynamics

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CHUN, Taewoo, PATTEM, Jacob, GILLIS, Richard B., DINU, Vlad T., YAKUBOV, Gleb E., CORFIELD, Anthony P. and HARDING, Stephen E. (2023). Self-association of the glycopeptide antibiotic teicoplanin A2 in aqueous solution studied by molecular hydrodynamics. Scientific Reports, 13 (1): 1969.

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Official URL: http://dx.doi.org/10.1038/s41598-023-28740-8
Open Access URL: https://www.nature.com/articles/s41598-023-28740-8... (Published version)
Link to published version:: https://doi.org/10.1038/s41598-023-28740-8
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    Abstract

    <jats:title>Abstract</jats:title><jats:p>The natural glycopeptide antibiotic teicoplanin is used for the treatment of serious Gram-positive related bacterial infections and can be administered intravenously, intramuscularly, topically (ocular infections), or orally. It has also been considered for targeting viral infection by SARS-CoV-2. The hydrodynamic properties of teicoplanin A2 (<jats:italic>M</jats:italic><jats:sub>1</jats:sub> = 1880 g/mol) were examined in phosphate chloride buffer (pH 6.8, <jats:italic>I</jats:italic> = 0.10 M) using sedimentation velocity and sedimentation equilibrium in the analytical ultracentrifuge together with capillary (rolling ball) viscometry. In the concentration range, 0–10 mg/mL teicoplanin A2 was found to self-associate plateauing &gt; 1 mg/mL to give a molar mass of (35,400 ± 1000) g/mol corresponding to ~ (19 ± 1) mers, with a sedimentation coefficient <jats:italic>s</jats:italic><jats:sub>20, w</jats:sub> =  ~ 4.65 S. The intrinsic viscosity [<jats:inline-formula><jats:alternatives><jats:tex-math>$$\eta$$</jats:tex-math><mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"> <mml:mi>η</mml:mi> </mml:math></jats:alternatives></jats:inline-formula>] was found to be (3.2 ± 0.1) mL/g: both this, the value for <jats:italic>s</jats:italic><jats:sub>20,w</jats:sub> and the hydrodynamic radius from dynamic light scattering are consistent with a globular macromolecular assembly, with a swelling ratio through dynamic hydration processes of ~ 2. </jats:p>

    Item Type: Article
    Identification Number: https://doi.org/10.1038/s41598-023-28740-8
    SWORD Depositor: Symplectic Elements
    Depositing User: Symplectic Elements
    Date Deposited: 06 Feb 2023 11:40
    Last Modified: 06 Feb 2023 12:21
    URI: https://shura.shu.ac.uk/id/eprint/31412

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