Self-association of the glycopeptide antibiotic teicoplanin A2 in aqueous solution studied by molecular hydrodynamics

CHUN, Taewoo, PATTEM, Jacob, GILLIS, Richard B., DINU, Vlad T., YAKUBOV, Gleb E., CORFIELD, Anthony P. and HARDING, Stephen E. (2023). Self-association of the glycopeptide antibiotic teicoplanin A2 in aqueous solution studied by molecular hydrodynamics. Scientific Reports, 13 (1): 1969.

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Official URL: http://dx.doi.org/10.1038/s41598-023-28740-8
Open Access URL: https://www.nature.com/articles/s41598-023-28740-8... (Published version)
Link to published version:: https://doi.org/10.1038/s41598-023-28740-8
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    Abstract

    <jats:title>Abstract</jats:title><jats:p>The natural glycopeptide antibiotic teicoplanin is used for the treatment of serious Gram-positive related bacterial infections and can be administered intravenously, intramuscularly, topically (ocular infections), or orally. It has also been considered for targeting viral infection by SARS-CoV-2. The hydrodynamic properties of teicoplanin A2 (<jats:italic>M</jats:italic><jats:sub>1</jats:sub> = 1880 g/mol) were examined in phosphate chloride buffer (pH 6.8, <jats:italic>I</jats:italic> = 0.10 M) using sedimentation velocity and sedimentation equilibrium in the analytical ultracentrifuge together with capillary (rolling ball) viscometry. In the concentration range, 0–10 mg/mL teicoplanin A2 was found to self-associate plateauing &gt; 1 mg/mL to give a molar mass of (35,400 ± 1000) g/mol corresponding to ~ (19 ± 1) mers, with a sedimentation coefficient <jats:italic>s</jats:italic><jats:sub>20, w</jats:sub> =  ~ 4.65 S. The intrinsic viscosity [<jats:inline-formula><jats:alternatives><jats:tex-math>$$\eta$$</jats:tex-math><mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"> <mml:mi>η</mml:mi> </mml:math></jats:alternatives></jats:inline-formula>] was found to be (3.2 ± 0.1) mL/g: both this, the value for <jats:italic>s</jats:italic><jats:sub>20,w</jats:sub> and the hydrodynamic radius from dynamic light scattering are consistent with a globular macromolecular assembly, with a swelling ratio through dynamic hydration processes of ~ 2. </jats:p>

    Item Type: Article
    Identification Number: https://doi.org/10.1038/s41598-023-28740-8
    SWORD Depositor: Symplectic Elements
    Depositing User: Symplectic Elements
    Date Deposited: 06 Feb 2023 11:40
    Last Modified: 06 Feb 2023 12:21
    URI: https://shura.shu.ac.uk/id/eprint/31412

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