Controlled depolymerisation assessed by analytical ultracentrifugation of low molecular weight chitosan for use in archaeological conservation

WAKEFIELD, JMK, GILLIS, RB, ADAMS, GG, MCQUEEN, C and HARDING, SE (2018). Controlled depolymerisation assessed by analytical ultracentrifugation of low molecular weight chitosan for use in archaeological conservation. European Biophysics Journal, 47, 769-775.

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Official URL: https://link.springer.com/article/10.1007/s00249-0...
Open Access URL: https://link.springer.com/content/pdf/10.1007/s002... (Published)
Link to published version:: https://doi.org/10.1007/s00249-018-1290-6

Abstract

The heterogeneity and molecular weight of a chitosan of low molecular weight (molar mass) and low degree of acetylation (0.1) for potential use as a consolidant for decayed archaeological wood were examined by sedimentation velocity and sedimentation equilibrium in the analytical ultracentrifuge before and after depolymerisation. Sedimentation velocity before depolymerisation revealed a uniform distribution of sedimentation coefficient with little concentration dependence. SEDFIT-MSTAR analysis revealed a weight average molecular weight Mw of (14.2 ± 1.2) kDa, and polydispersity index of ~ 1.2. Further analysis using MULTISIG revealed a distribution of material between 2 and 20 kDa and consistent with the weight average Mw. Controlled depolymerisation using hydrogen peroxide and ultra-violet radiation in an acetic acid medium reduced this to (4.9 ± 0.7) kDa, with a similar polydispersity. The depolymerised material appears to be within the range that has been predicted to fully penetrate into archaeological wood. The consequences for this finding and the use of the analytical ultracentrifuge in wood conservation strategies are considered.

Item Type: Article
Uncontrolled Keywords: Biophysics; 0202 Atomic, Molecular, Nuclear, Particle and Plasma Physics; 0299 Other Physical Sciences; 0601 Biochemistry and Cell Biology
Identification Number: https://doi.org/10.1007/s00249-018-1290-6
Page Range: 769-775
SWORD Depositor: Symplectic Elements
Depositing User: Symplectic Elements
Date Deposited: 03 May 2023 12:04
Last Modified: 03 May 2023 12:04
URI: https://shura.shu.ac.uk/id/eprint/31144

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