In depth investigation of the capabilities and limitations of combined proteomic-MALDI MS based approach for the forensic detection of blood

KENNEDY, Katie, GANNICLIFFE, Chris, COLE, Laura, SEALEY, Mark and FRANCESE, Simona (2022). In depth investigation of the capabilities and limitations of combined proteomic-MALDI MS based approach for the forensic detection of blood. Science & Justice, 62 (5), 602-609.

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Official URL: https://www.sciencedirect.com/science/article/pii/...
Open Access URL: https://doi.org/10.1016/j.scijus.2022.09.001 (Published version)
Link to published version:: https://doi.org/10.1016/j.scijus.2022.09.001

Abstract

For the past 7 years, Matrix Assisted Laser Desorption Ionisation Mass Spectrometry (MALDI MS) based methods have been developed and published for the forensic detection of blood in stains and fingermarks. However, in the view of adoption in an operational context, further investigation into the capabilities and limitations of this approach must be conducted. The refinement and testing of this approach must also be tailored to the requirements of the end users, enabling them to address the specific circumstances most encountered in a forensic scenario. The present study delves deeper into the assessment of the applicability of MALDI MS based strategy for the reliable and robust detection of human blood through: (i) a semi-qualitative assessment of the sensitivity of the method, (ii) a wider investigation of the compatibility of the method with the prior application of commonly used presumptive tests and (iii) assessment of the specificity of the method (when blood is present in mixture with other biofluids) and of its robustness, by assessing blood detection from a range of porous materials. The findings strengthen the evidence supporting the adoption of MALDI MS based approaches as a confirmatory test for the forensic detection of human blood in an operational context.

Item Type: Article
Uncontrolled Keywords: Blood; Forensic; Validation; MALDI; Compatibility; Biofluids; Legal & Forensic Medicine
Identification Number: https://doi.org/10.1016/j.scijus.2022.09.001
Page Range: 602-609
SWORD Depositor: Symplectic Elements
Depositing User: Symplectic Elements
Date Deposited: 25 Oct 2022 16:20
Last Modified: 12 Oct 2023 09:31
URI: https://shura.shu.ac.uk/id/eprint/30941

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