TRIM, P. J., DJIDJA, M. C., ATKINSON, S. J., OAKES, K., COLE, L. M., ANDERSON, D. M. G., HART, P. J., FRANCESE, S. and CLENCH, M. R. (2010). Introduction of a 20 kHz Nd:YVO4 laser into a hybrid quadrupole time-of-flight mass spectrometer for MALDI-MS imaging. Analytical and Bioanalytical Chemistry, 397 (8), 3409-3419.
Full text not available from this repository.Abstract
A commercial hybrid quadrupole time-of-flight mass spectrometer has been modified for high-speed matrix-assisted laser desorption ionisation (MALDI) imaging using a short-pulse optical technology Nd:YVO4 laser. The laser operating in frequency-tripled mode (lambda=355 nm) is capable of delivering 1.5-ns pulses of energy at up to 8 mu J at 510 kHz and 3 mu J at 20 kHz. Experiments to improve beam homogeneity and reduce laser speckle by mechanical vibration of the fibre-optic laser delivery system are reported along with data from trial and tissue imaging experiments using the modified instrument. The laser appeared to yield best results for MALDI-MS imaging experiments when operating at repetition rates 5-10 kHz. Combining this with raster imaging allowed images of rat brain sections to be recorded in 37 min. Similarly, images of the distribution of peptides in "on-tissue" digest experiments from tumour tissues were recorded in 1 h and 30 min rather than the 8h acquisition time previously used. A brief investigation of targeted protein analysis/imaging by multiple reaction monitoring experiments "on-tissue" is reported. A total of 26 transitions were recorded over a 3-s cycle time and images of abundant proteins were successfully recorded.
Item Type: | Article |
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Research Institute, Centre or Group - Does NOT include content added after October 2018: | Biomedical Research Centre |
Identification Number: | https://doi.org/10.1007/s00216-010-3874-6 |
Page Range: | 3409-3419 |
Depositing User: | Users 4 not found. |
Date Deposited: | 21 Jan 2011 14:40 |
Last Modified: | 18 Mar 2021 21:00 |
URI: | https://shura.shu.ac.uk/id/eprint/2991 |
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