Multi-Modal Mass Spectrometric Imaging of Uveal Melanoma

COLE, Laura M., HANDLEY, Joshua, CLAUDE, Emmanuelle, DUCKETT, Catherine J., MUDHAR, Hardeep S., SISLEY, Karen and CLENCH, Malcolm R. (2021). Multi-Modal Mass Spectrometric Imaging of Uveal Melanoma. Metabolites, 11 (8).

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Abstract

Matrix assisted laser desorption ionisation mass spectrometry imaging (MALDI-MSI), was used to obtain images of lipids and metabolite distribution in formalin fixed and embedded in paraffin (FFPE) whole eye sections containing primary uveal melanomas (UM). Using this technique, it was possible to obtain images of lysophosphatidylcholine (LPC) type lipid distribution that highlighted the tumour regions. Laser ablation inductively coupled plasma mass spectrometry images (LA-ICP-MS) performed on UM sections showed increases in copper within the tumour periphery and intratumoural zinc in tissue from patients with poor prognosis. These preliminary data indicate that multi-modal MSI has the potential to provide insights into the role of trace metals and cancer metastasis.

Item Type:	Article
Additional Information:	** From MDPI via Jisc Publications Router ** Licence for this article: https://creativecommons.org/licenses/by/4.0/ **Journal IDs: eissn 2218-1989 **History: published 23-08-2021; accepted 18-08-2021
Uncontrolled Keywords:	uveal melanoma mass spectrometry imaging, matrix assisted laser desorption ionisation, laser ablation inductively coupled plasma, cancer progression, genetic biomarkers, heterogeneity
Identification Number:	https://doi.org/10.3390/metabo11080560
SWORD Depositor:	Colin Knott
Depositing User:	Colin Knott
Date Deposited:	03 Sep 2021 12:49
Last Modified:	03 Sep 2021 13:00
URI:	https://shura.shu.ac.uk/id/eprint/28972

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