Visualizing Cholesterol in the Brain by On-Tissue Derivatization and Quantitative Mass Spectrometry Imaging.

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ANGELINI, Roberto, YUTUC, Eylan, WYATT, Mark F., NEWTON, Jillian, YUSUF, Fowzi A., GRIFFITHS, Lauren, COOZE, Benjamin J., EL ASSAD, Dana, FRACHE, Gilles, RAO, Wei, ALLEN, Luke B., KORADE, Zeljka, NGUYEN, Thu T.A., RATHNAYAKE, Rathnayake A.C., COLOGNA, Stephanie M., HOWELL, Owain W., CLENCH, Malcolm R, WANG, Yuqin and GRIFFITHS, William J (2021). Visualizing Cholesterol in the Brain by On-Tissue Derivatization and Quantitative Mass Spectrometry Imaging. Analytical Chemistry. [Article]

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Abstract
Despite being a critical molecule in the brain, mass spectrometry imaging (MSI) of cholesterol has been under-reported compared to other lipids due to the difficulty in ionizing the sterol molecule. In the present work, we have employed an on-tissue enzyme-assisted derivatization strategy to improve detection of cholesterol in brain tissue sections. We report distribution and levels of cholesterol across specific structures of the mouse brain, in a model of Niemann-Pick type C1 disease, and during brain development. MSI revealed that in the adult mouse, cholesterol is the highest in the pons and medulla and how its distribution changes during development. Cholesterol was significantly reduced in the corpus callosum and other brain regions in the Npc1 null mouse, confirming hypomyelination at the molecular level. Our study demonstrates the potential of MSI to the study of sterols in neuroscience.
More Information
Open Access URL:
https://pubs.acs.org/doi/pdf/10.1021/acs.analchem....
Open Access Version:
Published version
Uncontrolled Keywords:
Analytical Chemistry; 0301 Analytical Chemistry; 0399 Other Chemical Sciences
Event Location:
United States

Identifiers

Identification Number:
10.1021/acs.analchem.0c05399
ORCID for Mark F. Wyatt: ORCID iD orcid.org/0000-0003-4107-5941
ORCID for Zeljka Korade: ORCID iD orcid.org/0000-0002-8690-4507
ORCID for Stephanie M. Cologna: ORCID iD orcid.org/0000-0002-3541-3361
ORCID for Malcolm R Clench: ORCID iD orcid.org/0000-0002-0798-831X
ORCID for William J Griffiths: ORCID iD orcid.org/0000-0002-4129-6616

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Item Type:
Article
SWORD Depositor:
Symplectic Elements
Depositing User:
Symplectic Elements
Date Deposited:
19 Mar 2021 11:49
Last Modified:
18 Dec 2024 20:33
Date of first compliant deposit:
18 March 2021
Date of first compliant Open Access:
19 March 2021
Version of first compliant deposit:
Version of Record
URI:
https://shura.shu.ac.uk/id/eprint/28419
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