Dynamic cycling of eIF2 through a large eIF2B-containing cytoplasmic body

CAMPBELL, Susan, HOYLE, Nathaniel P. and ASHE, Mark P. (2005). Dynamic cycling of eIF2 through a large eIF2B-containing cytoplasmic body. Journal of Cell Biology, 170 (6), 925-934.

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Link to published version:: https://doi.org/10.1083/jcb.200503162


The eukaryotic translation initiation factor 2B (eIF2B) provides a fundamental controlled point in the pathway of protein synthesis. eIF2B is the heteropentameric guanine nucleotide exchange factor that converts eIF2, from an inactive guanosine diphosphate–bound complex to eIF2-guanosine triphosphate. This reaction is controlled in response to a variety of cellular stresses to allow the rapid reprogramming of cellular gene expression. Here we demonstrate that in contrast to other translation initiation factors, eIF2B and eIF2 colocalize to a specific cytoplasmic locus. The dynamic nature of this locus is revealed through fluorescence recovery after photobleaching analysis. Indeed eIF2 shuttles into these foci whereas eIF2B remains largely resident. Three different strategies to decrease the guanine nucleotide exchange function of eIF2B all inhibit eIF2 shuttling into the foci. These results implicate a defined cytoplasmic center of eIF2B in the exchange of guanine nucleotides on the eIF2 translation initiation factor. A focused core of eIF2B guanine nucleotide exchange might allow either greater activity or control of this elementary conserved step in the translation pathway.

Item Type: Article
Uncontrolled Keywords: 06 Biological Sciences; 11 Medical and Health Sciences; Developmental Biology
Identification Number: https://doi.org/10.1083/jcb.200503162
Page Range: 925-934
SWORD Depositor: Symplectic Elements
Depositing User: Symplectic Elements
Date Deposited: 12 Apr 2021 15:24
Last Modified: 12 Apr 2021 15:30
URI: https://shura.shu.ac.uk/id/eprint/28064

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