Development of in vitro assays for the detection of botulinum toxins in foods

NEWTON, Kirsti, WICTOME, Matthew, JAMESON, Karen, DUNNIGAN, Paul, CLARKE, Sally, GAZE, Joy, TAUK, Annie, FOSTER, Keith and SHONE, Cifford (1999). Development of in vitro assays for the detection of botulinum toxins in foods. FEMS Immunology and Medical Microbiology, 24 (3), 319-323.

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Currently the only accepted method for the detection of botulinum neurotoxin in contaminated samples is the mouse bioassay. Although highly sensitive this test has a number of drawbacks: it is expensive to perform, lacks specificity and involves the use of animals. With increasing resistance to such animal tests there is a need to replace the bioassay with a reliable in vitro test. Over the past six years it has been demonstrated that all the botulinum neurotoxins act intracellularly as highly specific zinc endoproteases, cleaving proteins involved in the control of secretion of neurotransmitters. In the work described, this enzymatic activity has been utilised in assay formats for the detection in foods of neurotoxin of the serotypes involved in food-borne outbreaks in man. These assays have been shown to have a greater sensitivity, speed and specificity than the mouse bioassay. It is envisaged that such assays will prove realistic alternatives to animal-based tests.

Item Type: Article
Uncontrolled Keywords: 1102 Cardiorespiratory Medicine and Haematology; 1107 Immunology; 1108 Medical Microbiology; Microbiology
Identification Number:
Page Range: 319-323
SWORD Depositor: Symplectic Elements
Depositing User: Symplectic Elements
Date Deposited: 12 Apr 2021 14:55
Last Modified: 12 Apr 2021 14:55

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