Pre-validation of a MALDI MS proteomics-based method for the reliable detection of blood and blood provenance

KENNEDY, Katie, HEATON, Cameron, LANGENBURG, Glenn, COLE, Laura, CLARK, Tom, CLENCH, Malcolm R., SEARS, Vaughn, SEALEY, Mark, MCCOLM, Richard and FRANCESE, Simona (2020). Pre-validation of a MALDI MS proteomics-based method for the reliable detection of blood and blood provenance. Scientific Reports, 10 (1), p. 17087.

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Abstract: The reliable identification of blood, as well as the determination of its origin (human or animal) is of great importance in a forensic investigation. Whilst presumptive tests are rapid and deployed in situ, their very nature requires confirmatory tests to be performed remotely. However, only serological tests can determine blood provenance. The present study improves on a previously devised Matrix Assisted Laser Desorption Ionisation Mass Spectrometry (MALDI MS)—proteomics based method for the reliable detection of blood by enabling the determination of blood provenance. The overall protocol was developed to be more specific than presumptive tests and faster/easier than the gold standard liquid chromatography (LC) MS/MS analysis. This is considered a pre-validation study that has investigated stains and fingermarks made in blood, other biofluids and substances that can elicit a false-positive response to colorimetric or presumptive tests, in a blind fashion. Stains and marks were either untreated or enhanced with a range of presumptive tests. Human and animal blood were correctly discriminated from other biofluids and non-biofluid related matrices; animal species determination was also possible within the system investigated. The procedure is compatible with the prior application of presumptive tests. The refined strategy resulting from iterative improvements through a trial and error study of 56 samples was applied to a final set of 13 blind samples. This final study yielded 12/13 correct identifications with the 13th sample being correctly identified as animal blood but with no species attribution. This body of work will contribute towards the validation of MALDI MS based methods and deployment in violent crimes involving bloodshed.

Item Type: Article
Additional Information: ** From Springer Nature via Jisc Publications Router ** Licence for this article: **Journal IDs: eissn 2045-2322 **Article IDs: publisher-id: s41598-020-74253-z; manuscript: 74253 **History: collection 12-2020; online 13-10-2020; published_online 13-10-2020; registration 30-09-2020; accepted 28-09-2020; submitted 15-04-2020
Uncontrolled Keywords: Article, /631/1647, /692/53, article
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Page Range: p. 17087
SWORD Depositor: Colin Knott
Depositing User: Colin Knott
Date Deposited: 20 Oct 2020 11:33
Last Modified: 17 Mar 2021 21:48

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