Mass spectrometry imaging of endogenous metabolites in response to doxorubicin in a novel 3D osteosarcoma cell culture model

PALUBECKAITĖ, Ieva, CROOKS, Lucy, SMITH, David, COLE, Laura, BRAM, Heijs, LE MAITRE, Christine, CLENCH, Malcolm and CROSS, Neil A (2019). Mass spectrometry imaging of endogenous metabolites in response to doxorubicin in a novel 3D osteosarcoma cell culture model. Journal of Mass Spectrometry.

Palubeckaite 2019 submitted to JMS 10 2019.pdf - Accepted Version
All rights reserved.

Download (720kB) | Preview
Official URL:
Link to published version::


Three‐dimensional (3D) cell culture is a rapidly emerging field which mimics some of the physiological conditions of human tissues. In cancer biology, it is considered a useful tool in predicting in vivo chemotherapy responses compared with conventional two‐dimensional cell culture. We have developed a novel 3D cell culture model of osteosarcoma comprised of aggregated proliferative tumour spheroids, which shows regions of tumour heterogeneity formed by aggregated spheroids of polyclonal tumour cells. Aggregated spheroids show local necrotic and apoptotic regions, and have sizes suitable for the study of spatial distribution of metabolites by mass spectrometry imaging (MSI). We have used this model to perform a proof‐of‐principle study showing a heterogeneous distribution of endogenous metabolites that co‐localise with the necrotic core and apoptotic regions in this model. Cytotoxic chemotherapy (Doxorubicin) responses were significantly attenuated in our 3D cell culture model compared with standard cell culture, as determined by Resazurin assay, despite sufficient doxorubicin diffusion demonstrated by localisation throughout the 3D constructs. Finally, changes to the distribution of endogenous metabolites in response to Doxorubicin were readily detected by MSI. Principle component analysis identified 50 metabolites which differed most in their abundance between treatment groups, and of these, 10 were identified by both in‐software t test and mixed effects ANOVA. Subsequent independent MSI of identified species were consistent with principle component analysis findings. This proof‐of‐principle study shows for the first time that chemotherapy‐induced changes in metabolite abundance and distribution may be determined in 3D cell culture by MSI, highlighting this method as a potentially useful tool in elucidation of chemotherapy responses as an alternative to in vivo testing.

Item Type: Article
Uncontrolled Keywords: Analytical Chemistry; 03 Chemical Sciences
Identification Number:
SWORD Depositor: Symplectic Elements
Depositing User: Symplectic Elements
Date Deposited: 28 Oct 2019 15:53
Last Modified: 17 Mar 2021 21:31

Actions (login required)

View Item View Item


Downloads per month over past year

View more statistics