Cytotoxic effects of Smp24 and Smp43 scorpion venom antimicrobial peptides on tumour and non-tumour cell lines

ELRAYESS, Ranwa A., MOHALLAL, Mahmoud E, EL-SHAHAT, Yomn M, EBAID, Hala M, MILLER, Keith, STRONG, Peter and ABDEL-RAHMAN, Mohamed A (2019). Cytotoxic effects of Smp24 and Smp43 scorpion venom antimicrobial peptides on tumour and non-tumour cell lines. International Journal of Peptide Research and Therapeutics, 26 (3), 1409-1415. [Article]

Documents
25299:538428
[thumbnail of Elrayess2019_Article_CytotoxicEffectsOfSmp24AndSmp4.pdf]
Preview
PDF
Elrayess2019_Article_CytotoxicEffectsOfSmp24AndSmp4.pdf - Published Version
Available under License Creative Commons Attribution.

Download (1MB) | Preview
Abstract
Smp24 and Smp43 are novel cationic AMPs identified from the venom of the Egyptian scorpion Scorpio maurus palmatus, having potent activity against both Gram-positive and Gram-negative bacteria as well as fungi. Here we describe cytotoxicity of these peptides towards three non-tumour cell lines (CD34+ (hematopoietic stem progenitor from cord blood), HRECs (human renal epithelial cells) and HACAT (human skin keratinocytes) and two acute leukaemia cell lines (myeloid (KG1a) and lymphoid (CCRF-CEM) leukaemia cell lines) using a combination of biochemical and imaging techniques. Smp24 and Smp43 (4–256 µg/mL) decreased the cell viability (as measured by intracellular ATP) of all cells tested, although keratinocytes were markedly less sensitive. Cell membrane leakage as evidenced by the release of lactate dehydrogenase was evident throughout and was confirmed by scanning electron microscope studies.
More Information
Statistics

Downloads

Downloads per month over past year

View more statistics

Metrics

Altmetric Badge

Dimensions Badge

Share
Add to AnyAdd to TwitterAdd to FacebookAdd to LinkedinAdd to PinterestAdd to Email

Actions (login required)

View Item View Item