Lipid coated liquid crystal droplets for the on-chip detection of antimicrobial peptides

BAO, Peng, PATERSON, Daniel A., HARRISON, Patrick L., MILLER, Keith, PEYMAN, Sally, JONES, J. Cliff, SANDOE, Jonathan, EVANS, Stephen D., BUSHBY, Richard J. and GLEESON, Helen F. (2019). Lipid coated liquid crystal droplets for the on-chip detection of antimicrobial peptides. Lab on a chip, 19 (6), 1082-1089.

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We describe a novel biosensor based on phospholipid-coated nematic liquid crystal (LC) droplets and demonstrate the detection of Smp43, a model antimicrobial peptide (AMP) from the venom of North African scorpion Scorpio maurus palmatus. Mono-disperse lipid-coated LC droplets of diameter 16.7 ± 0.2 μm were generated using PDMS microfluidic devices with a flow-focusing configuration and were the target for AMPs. The droplets were trapped in a bespoke microfluidic trap structure and were simultaneously treated with Smp43 at gradient concentrations in six different chambers. The disruption of the lipid monolayer by the Smp43 was detected (<6 μM) at concentrations well within its biologically active range, indicated by a dramatic change in the appearance of the droplets associated with the transition from a typical radial configuration to a bipolar configuration, which is readily observed by polarizing microscopy. This suggests the system has feasibility as a drug-discovery screening tool. Further, compared to previously reported LC droplet biosensors, this LC droplet biosensor with a lipid coating is more biologically relevant and its ease of use in detecting membrane-related biological processes and interactions has the potential for development as a reliable, low-cost and disposable point of care diagnostic tool.

Item Type: Article
Additional Information: ** Article version: VoR ** From Crossref via Jisc Publications Router ** Licence for VoR version of this article starting on 20-02-2019: **Journal IDs: pissn 1473-0197; eissn 1473-0189 **History: published 2019
Uncontrolled Keywords: Biochemistry, Bioengineering, General Chemistry, Biomedical Engineering
Identification Number:
Page Range: 1082-1089
SWORD Depositor: Louise Beirne
Depositing User: Louise Beirne
Date Deposited: 01 Mar 2019 09:53
Last Modified: 18 Mar 2021 06:22

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