MALDI-MSI for the analysis of a 3D tissue-engineered psoriatic skin model

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HARVEY, Amanda, DAY, Rebecca, COLE, Laura M., BARTLETT, Maggie, WARWICK, John, BOJAR, Richard, SMITH, David, CROSS, Neil and CLENCH, Malcolm R. (2016). MALDI-MSI for the analysis of a 3D tissue-engineered psoriatic skin model. Proteomics, 16 (11-12), 1718-1725. [Article]

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Abstract
MALDI-MS Imaging is a novel label-free technique that can be used to visualize the changes in multiple mass responses following treatment. Following treatment with proinflammatory cytokine interleukin-22 (IL-22), the epidermal differentiation of Labskin, a living skin equivalent (LSE), successfully modeled psoriasis in vitro. Masson’s trichrome staining enabled visualization and quantification of epidermal differentiation between the untreated and IL-22 treated psoriatic LSEs. Matrix-assisted laser desorption ionization mass spectrometry imaging was used to observe the spatial location of the psoriatic therapy drug acetretin following 48 h treatments within both psoriatic and normal LSEs. After 24 h, the drug was primarily located in the epidermal regions of both the psoriatic and nonpsoriatic LSE models whereas after 48 h it was detectible in the dermis.
More Information
Official URL:
http://onlinelibrary.wiley.com/doi/10.1002/pmic.20...
Research Institute, Centre or Group - Does NOT include content added after October 2018:
Biomedical Research Centre
Page Range:
1718-1725

Identifiers

Identification Number:
10.1002/pmic.201600036
ORCID for Amanda Harvey: ORCID iD orcid.org/0000-0003-3720-2602
ORCID for Laura M. Cole: ORCID iD orcid.org/0000-0002-2538-6291
ORCID for David Smith: ORCID iD orcid.org/0000-0001-5177-8574
ORCID for Neil Cross: ORCID iD orcid.org/0000-0003-2055-5815
ORCID for Malcolm R. Clench: ORCID iD orcid.org/0000-0002-0798-831X

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Item Type:
Article
Depositing User:
Users 3084 not found.
Date Deposited:
27 Jul 2016 13:39
Last Modified:
18 Dec 2024 23:55
Date of first compliant deposit:
27 July 2016
Date of first compliant Open Access:
27 July 2016
Version of first compliant deposit:
Version of Record
URI:
https://shura.shu.ac.uk/id/eprint/12690
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