HURST, Louise A., BUNNING, Rowena A.D., SHARRACK, Basil and WOODROOFE, M. Nicola (2012). siRNA knockdown of ADAM-10, but not ADAM-17, significantly reduces fractalkine shedding following pro-inflammatory cytokine treatment in a human adult brain endothelial cell line. Neuroscience Letters, 521 (1), 52-56.
Full text not available from this repository.Abstract
Fractalkine shedding is believed to occur constitutively and following induction via the activity of two membrane-bound enzymes, ADAM-10 and ADAM-17. However, our previous work suggested that ADAM-17 is not involved in the proteolytic release of fractalkine under TNF treatment of a human adult brain endothelial cell line, hCMEC/D3. The pro-inflammatory cytokine, TNF, has previously been shown to be expressed in the perivascular cuffs in multiple sclerosis. Here we sought to identify, using siRNAs to silence the expression of ADAM-10 and ADAM-17, whether ADAM-10 is responsible for TNF-induced shedding of fractalkine from the cell membrane in hCMEC/D3. Our findings suggest that ADAM-10, and not ADAM-17, is the major protease involved in fractalkine release under pro-inflammatory conditions in this human adult brain endothelial cell model.
Item Type: | Article |
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Research Institute, Centre or Group - Does NOT include content added after October 2018: | Biomedical Research Centre |
Identification Number: | https://doi.org/10.1016/j.neulet.2012.05.057 |
Page Range: | 52-56 |
Depositing User: | Users 3084 not found. |
Date Deposited: | 13 Dec 2012 09:50 |
Last Modified: | 18 Mar 2021 20:00 |
URI: | https://shura.shu.ac.uk/id/eprint/6550 |
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