Stabilization of neurotoxic soluble beta-sheet-rich conformations of the Alzheimer's disease amyloid-beta peptide.

TEW, Deborah J, BOTTOMLEY, Stephen P, SMITH, David P, CICCOTOSTO, Giuseppe D, BABON, Jeffrey, HINDS, Mark G, MASTERS, Colin L, CAPPAI, Roberto and BARNHAM, Kevin J (2008). Stabilization of neurotoxic soluble beta-sheet-rich conformations of the Alzheimer's disease amyloid-beta peptide. Biophysical journal, 94 (7), 2752-2766.

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Link to published version:: 10.1529/biophysj.107.119909

Abstract

An emerging paradigm for degenerative diseases associated with protein misfolding, such as Alzheimer's disease, is the formation of a toxic species due to structural transitions accompanied by oligomerization. Increasingly, the focus in Alzheimer's disease is on soluble oligomeric forms of the amyloid-beta peptide (Abeta) as the potential toxic species. Using a variety of methods, we have analyzed how sodium dodecyl sulphate (SDS) modulates the folding of Abeta40 and 42 and found that submicellar concentrations of SDS solubilize Abeta and induce structural transitions. Under these conditions, Abeta40 and 42 are interconverting oligomeric ensembles with a predominantly beta-sheet structure. The Abeta42 soluble oligomers form beta-sheet structures more readily and have increased stability compared with Abeta40 under identical conditions. The presence of added Cu(2+) significantly promotes and stabilizes the formation of the soluble oligomeric beta-sheet structures but these structures are nonamyloidogenic. In contrast, in the absence of added Cu(2+), these beta-sheet oligomers possess the hallmarks of amyloidogenic structures. These SDS-induced beta-sheet forms of Abeta, both in the presence and absence of Cu(2+), are toxic to neuronal cells.

Item Type: Article
Research Institute, Centre or Group: Biomolecular Sciences Research Centre
Identification Number: 10.1529/biophysj.107.119909
Depositing User: Jamie Young
Date Deposited: 03 Jun 2015 10:41
Last Modified: 03 Jun 2015 10:41
URI: http://shura.shu.ac.uk/id/eprint/9994

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