Antimicrobial susceptibility testing of Chlamydia trachomatis using a reverse transcriptase PCR-based method.

CROSS, N A, KELLOCK, D J, KINGHORN, G R, TARAKTCHOGLOU, M, BATAKI, E, OXLEY, K M, HAWKEY, P M and ELEY, A (1999). Antimicrobial susceptibility testing of Chlamydia trachomatis using a reverse transcriptase PCR-based method. Antimicrobial agents and chemotherapy, 43 (9), 2311-2313.

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Official URL: http://aac.asm.org/content/43/9/2311

Abstract

The conventional method for antimicrobial susceptibility testing of Chlamydia trachomatis is subjective and potentially misleading. We have developed a reverse transcriptase PCR (RT-PCR)-based method which is more sensitive and less subjective than the conventional method. Using 16 strains of C. trachomatis in triplicate assays, we found the RT-PCR method consistently more sensitive than the conventional technique for all eight antimicrobials tested, with resultant MICs determined by RT-PCR ranging from 1.6-fold higher (erythromycin) to >/=195-fold higher (amoxicillin).

Item Type: Article
Research Institute, Centre or Group: Biomolecular Sciences Research Centre
Depositing User: Jamie Young
Date Deposited: 03 Jun 2015 08:14
Last Modified: 03 Jun 2015 08:14
URI: http://shura.shu.ac.uk/id/eprint/9968

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