Detection of the epidermal growth factor receptor, amphiregulin and epiregulin in formalin-fixed paraffin-embedded human placenta tissue by matrix-assisted laser desorption/ionization mass spectrometry imaging

MAHMOUD, Khaled, COLE, Laura, NEWTON, Jillian, MOHAMED, Sabah, AL-ENAZI, Maher, QIRKE, Phil and CLENCH, Malcolm (2013). Detection of the epidermal growth factor receptor, amphiregulin and epiregulin in formalin-fixed paraffin-embedded human placenta tissue by matrix-assisted laser desorption/ionization mass spectrometry imaging. European Journal of Mass Spectrometry, 19 (1), 17-28.

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Official URL: http://dx.doi.org/10.1255/ejms.1212
Link to published version:: 10.1255/ejms.1212

Abstract

The study of the expression and the tissue distribution of the tyrosine kinase drug-target EGFR is of interest in oncology as a marker of potential efficacy of treatment. It has been reported, however, that the response rates to anti-EGFR drugs are poorly linked to its expression. Clinical studies have also revealed a patient response correlation with the expression levels of two EGFR ligands; amphiregulin and epiregulin. Here we report the development of a matrix assisted laser desorption ionisation mass spectrometry imaging methodology for the study of EGFR, epiregulin and amphiregulin distribution in formalin fixed paraffin embedded Human placental tissue and a comparison to expression patterns obtained by immunohiostochemistry. Using on-tissue digests and imaging of specific peptides, the tissue distribution of these proteins has been obtained down to 30µm spatial resolution.

Keywords: MALDI, tissue imaging, recombinant cancer therapeutics, EGFR, amphiregulin, epiregulin, FFPE, placenta, Cetuximab, Panitumumab, immunohistochemistry, colorectal cancer, targeted drugs

Item Type: Article
Research Institute, Centre or Group: Biomolecular Sciences Research Centre
Identification Number: 10.1255/ejms.1212
Depositing User: Jamie Young
Date Deposited: 28 May 2015 10:16
Last Modified: 28 May 2015 10:16
URI: http://shura.shu.ac.uk/id/eprint/9895

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