Tools
Tools
DUCKETT, Catherine, MCCULLAGH, Michael, SMITH, Christopher and WILSON, Ian D (2015). The metabolism of 4-bromoaniline in the bile-cannulated rat: application of ICPMS (79/81Br), HPLC-ICPMS & HPLC-oaTOFMS. Xenobiotica, 45 (8), 672-680.
|
PDF
Duckett_et_al_-_metabolism_of_4_bronmoaniline.pdf - Published Version Creative Commons Attribution Non-commercial No Derivatives. Download (453kB) | Preview |
Abstract
1. An excretion balance study was performed following i.p. administration of 4-bromoaniline (50 mg kg−1) to bile-cannulated rats, using bromine-detected (79/81Br) ICPMS for quantification. Approximately 90% of the dose was recovered in urine (68.9 ± 3.6%) and bile (21.4 ± 1.4%) by 48 h post-administration.
2. HPLC-ICPMS (79/81Br) was used to selectively detect and profile the major urinary and biliary-excreted metabolites and determined that the 0–12 h urine contained at least 21 brominated metabolites with 19 bromine-containing peaks observed in the 6–12 h bile samples.
3. The urinary and biliary metabolites were subsequently profiled using HPLC-oaTOFMS. By exploiting the distinctive bromine isotope pattern ca. 60 brominated metabolites were detected in the urine in negative electrospray ionisation (ESI) mode while bile contained ca. 21.
4. While a large number of bromine-containing metabolites were detected, the profiles were dominated by a few major components with the bulk of the 4-bromoaniline-related material in urine accounted for by 4-bromoanaline O-sulfate (∼75% of the total by ICPMS, 84% by TOFMS). In bile a hydroxylated N-acetyl compound was the major metabolite detected, forming some ∼65% of the 4-bromoaniline-related material by ICPMS (37% by TOFMS).
| Item Type: | Article |
|---|---|
| Additional Information: | Posted online on April 2, 2015 |
| Research Institute, Centre or Group - Does NOT include content added after October 2018: | Biomedical Research Centre |
| Identification Number: | https://doi.org/10.3109/00498254.2015.1007491 |
| Page Range: | 672-680 |
| Depositing User: | Users 3084 not found. |
| Date Deposited: | 07 May 2015 08:59 |
| Last Modified: | 18 Mar 2021 04:39 |
| URI: | https://shura.shu.ac.uk/id/eprint/9649 |
Actions (login required)
 |
View Item |
Downloads
Downloads per month over past year