CHEN, W J, LOU, W Y, YU, C Y, WU, H, ZONG, M H and SMITH, Thomas (2012). Use of hydrophilic ionic liquids in a two-phase system to improve Mung bean epoxide hydrolases-mediated asymmetric hydrolysis of styrene oxide. Journal of Biotechnology, 162 (2-3), 183-190.Full text not available from this repository.
A comparative study was made of Mung bean epoxide hydrolases-catalyzed asymmetric hydrolysis of styrene oxide to (R)-1-phenyl-1,2-ethanediol in an n-hexane/buffer biphasic system containing various hydrophilic ionic liquids (ILs). Compared to the n-hexane/buffer biphasic system alone, addition of a small amount of hydrophilic ILs reduced the amount of non-enzymatic hydrolysis, and improved the reaction rate by up to 22%. The ILs with cation containing an alkanol group, namely [C2OHMIM][BF4] and [C2OHMIM][TfO], and the choline amino acid ILs [Ch][Arg] and [Ch][Pro] were found to be the most suitable co-solvents for the reaction, owing to their good biocompatibility with the enzyme, which led to high initial rates (0.99–1.25 μmol/min) and high product e.e.s (95%). When substrate concentration was around 30 mM, where optimal performance was observed with the IL-containing systems, the product e.e. was improved from 90% without ILs to ≥95% in the presence of ILs.
|Research Institute, Centre or Group:||Biomolecular Sciences Research Centre|
|Depositing User:||Louise Vickers|
|Date Deposited:||31 Oct 2014 10:21|
|Last Modified:||31 Oct 2014 10:21|
Actions (login required)
Downloads per month over past year