siRNA knockdown of ADAM-10, but not ADAM-17, significantly reduces fractalkine shedding following pro-inflammatory cytokine treatment in a human adult brain endothelial cell line

HURST, Louise A., BUNNING, Rowena, SHARRACK, Basil and WOODROOFE, Nicola (2012). siRNA knockdown of ADAM-10, but not ADAM-17, significantly reduces fractalkine shedding following pro-inflammatory cytokine treatment in a human adult brain endothelial cell line. Neuroscience Letters, 521, 52-56.

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Abstract

Fractalkine shedding is believed to occur constitutively and following induction via the activity of two membrane-bound enzymes, ADAM-10 and ADAM-17, respectively. However, our previous work suggested that ADAM-17 is not involved in the proteolytic release of fractalkine under TNF treatment of a human adult brain endothelial cell line, hCMEC/D3. The pro-inflammatory cytokine, TNF, has previously been shown to be expressed in the perivascular cuffs in multiple sclerosis. Here we sought to identify, using siRNAs to silence the expression of ADAM-10 and ADAM-17, whether ADAM-10 is responsible for TNF-induced shedding of fractalkine from the cell membrane in hCMEC/D3. Our findings suggest that ADAM-10, and not ADAM-17, is the major protease involved in fractalkine release under pro-inflammatory conditions in this human adult brain endothelial cell model.

Item Type:	Article
Research Institute, Centre or Group:	Biomedical Research Centre
Identification Number:	10.1016/j.neulet.2012.05.057
Depositing User:	Rebecca Jones
Date Deposited:	12 Jun 2012 10:38
Last Modified:	21 Mar 2013 12:14
URI:	http://shura.shu.ac.uk/id/eprint/5361

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