Matrix assisted laser desorption mass spectrometry imaging for the examination of imipramine absorption by straticell-RHE-EPI/001 an artificial model of the human epidermis.

AVERY, Jennie L., MCKEWEN, Andrew, FLINDERS, Bryn, FRANCESE, Simona and CLENCH, Malcolm (2011). Matrix assisted laser desorption mass spectrometry imaging for the examination of imipramine absorption by straticell-RHE-EPI/001 an artificial model of the human epidermis. Xenobiotica, 41 (8), pp 735-742.

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Link to published version:: 10.3109/00498254.2011.573015

Abstract

In order to assess the potential of matrix-assisted laser desorption mass spectrometry imaging for the examination of artificial skin models, the absorption of the tricyclic antidepressant imipramine into Straticell-RHE-EPI/001 an artificial model of the human epidermis has been studied. The presence of imipramine could be clearly discerned in treated samples by imaging the distribution of the protonated molecule at m/z 281.18 in samples taken 2 and 8 h after treatment. No clear evidence of biotransformation of imipramine in the artificial epidermal model was detected, although some signals that could potentially be assigned to the desmethyl metabolite were detected. Further work is required in order to investigate the reasons for the apparent low levels of metabolites detected.

Item Type: Article
Research Institute, Centre or Group: Biomedical Research Centre
Identification Number: 10.3109/00498254.2011.573015
Depositing User: Rebecca Jones
Date Deposited: 21 Feb 2012 15:19
Last Modified: 21 Feb 2012 15:19
URI: http://shura.shu.ac.uk/id/eprint/4384

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