Astrocyte and endothelial cell expression of ADAM 17 (TACE) in adult human CNS

GODDARD, D. R., BUNNING, R. A. D. and WOODROOFE, M. N. (2001). Astrocyte and endothelial cell expression of ADAM 17 (TACE) in adult human CNS. Glia, 34 (4), p. 267.

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Link to published version:: 10.1002/glia.1060

Abstract

ADAM17, also known as TACE, is an important sheddase for a number of proteins, including tumor necrosis factor-alpha (TNF-alpha), transforming growth factor-alpha (TGF-alpha),L-selectin, p75, and p55 TNF receptors, and interleukin-1 receptor II (IL-1RII). The presence of ADAM17 mRNA in adult mouse and rat CNS was recently reported (Karkkainen et al. Mol Cell Neurosci 15:547–560, 2000). However, the cellular origin of ADAM17 remains unknown. In this study, we have used an anti-ADAM17 antibody in an immunohistochemical study of its distribution in human adult CNS tissue. Cells with astrocytic and endothelial morphology were ADAM17-positive. This finding was further confirmed using double immunofluorescence with antibodies against GFAP and von Willebrand factor, which label astrocytes and endothelial cells, respectively. This study demonstrates that ADAM 17 is expressed by astrocytes and endothelial cells in normal brain tissue and may have a role in normal brain function.

Item Type: Article
Uncontrolled Keywords: glia, sheddase, TNF-alpha, protease, cytokine
Research Institute, Centre or Group: Biomedical Research Centre
Identification Number: 10.1002/glia.1060
Depositing User: Ann Betterton
Date Deposited: 22 Feb 2008
Last Modified: 09 Dec 2009 18:23
URI: http://shura.shu.ac.uk/id/eprint/414

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