Study of receptor-chaperone interactions using the optical technique of spectroscopic ellipsometry

KRIECHBAUMER, Verena, TSARGORODSKAYA, Anna, MUSTAFA, Mohd, VINOGRADOVA, Tatiana, LACEY, Joanne, SMITH, David, ABELL, Benjamin and NABOK, Alexei (2011). Study of receptor-chaperone interactions using the optical technique of spectroscopic ellipsometry. Biophysical Journal, 101 (2), 504-511.

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Official URL: http://dx.doi.org/10.1016/j.bpj.2011.06.011
Link to published version:: 10.1016/j.bpj.2011.06.011

Abstract

This work describes a detailed quantitative interaction study between the novel plastidial chaperone receptor OEP61 and isoforms of the chaperone types Hsp70 and Hsp90 using the optical method of total internal reflection ellipsometry (TIRE). The receptor OEP61 was electrostatically immobilized on a gold surface via an intermediate layer of polycations. The TIRE measurements allowed the evaluation of thickness changes in the adsorbed molecular layers as a result of chaperone binding to receptor proteins. Hsp70 chaperone isoforms but not Hsp90 were shown to be capable of binding OEP61. Dynamic TIRE measurements were carried out to evaluate the affinity constants of the above reactions and resulted in clear discrimination between specific and nonspecific binding of chaperones as well as differences in binding properties between the highly similar Hsp70 isoforms.

Item Type: Article
Research Institute, Centre or Group: Materials and Engineering Research Institute > Thin Films Research Centre > Electronic Materials and Sensors Research Group
Identification Number: 10.1016/j.bpj.2011.06.011
Depositing User: Helen Garner
Date Deposited: 24 Oct 2011 15:03
Last Modified: 09 Oct 2012 10:15
URI: http://shura.shu.ac.uk/id/eprint/3972

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