Impact of leptin deficiency on male tibia and vertebral body 3D bone architecture independent of changes in body weight.

WILLIAMSON, Alex, DA SILVA, Alexandre, DO CARMO, Jussara M., LE MAITRE, Christine, HALL, John E. and ABERDEIN, Nicola (2023). Impact of leptin deficiency on male tibia and vertebral body 3D bone architecture independent of changes in body weight. Physiological Reports, 11 (19).

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Abstract

Leptin an adipokine with potent effects on energy balance and body weight plays an important role in defining bone architecture in growing mammals. However,major changes in body weight can also influence morphology of trabecular and cortical bone. Therefore, we examined the impact of leptin deficiency on tibia and vertebral body 3D bone architecture independent of changes in body weight. Furthermore, advances in computational 3D image analysis suggest that average morphological values may mask regional specific differences in trabecular bone thickness. The study utilized leptin-deficient Ob/Ob mice (n=8) weight-paired to C57BL/6 (C57) control mice (n=8) which were split into either lean or obese groups for 24±2weeks. Whole tibias and L3 vertebrae were fixed before high resolution microcomputed tomography (μCT) scanning was performed. Leptin deficiency independent of body weight reduced tibia cortical bone volume, trabecular bone volume/tissue volume, number, and mineral density. Mean tibia trabecular thickness showed no significant differences between all groups; however, significant changes in trabecular thickness were found when analyzed by region. This study demonstrates that leptin deficiency significantly impacts tibia and vertebral body trabecular and cortical bone 3D architecture independent of changes in body weight.

Item Type:	Article
Uncontrolled Keywords:	0606 Physiology; 1103 Clinical Sciences; 1116 Medical Physiology; 3208 Medical physiology
Identification Number:	https://doi.org/10.14814/phy2.15832
SWORD Depositor:	Symplectic Elements
Depositing User:	Symplectic Elements
Date Deposited:	26 Sep 2023 08:24
Last Modified:	11 Oct 2023 10:53
URI:	https://shura.shu.ac.uk/id/eprint/32429

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